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Passive elution and electro elution are the most common methods for extracting proteins and/or nucleic acid

from polyacrylamide/agarose gels. Protein extraction by passive elution usually takes overnight incubation.

Eluted proteins are significantly diluted and require further concentration. Protein extraction by electro elution

is faster (30 min to 2 h) than passive elution but it requires a special elutiondevice that works less effectively

for larger proteins (>70 Kda). The electro buffer usually contains detergent and other chemicals at a

concentration that may interfere with downstream applications. Here we feature a rapid and instrument-free

protein/nucleic acid extraction kit. Proteins/nucleic acid can be extracted from gels in <10 min with high

yield. The elution buffer can be a detergent-containing buffer or pure water depending upon methods of gel

staining. The elution volume is between 10 to 200µl. Multiple gel pieces can be processed in a single tube

and the final protein concentration is relatively high.