Products

Protein

MEM-Myosin

Origin

Skeletal muscle, rabbit

Molecular mass

~480kDa

Protein description

NEM-Myosin II is chemically inactivated fast muscle myosin from rabbit psoas

muscle. Myosin II is a hexameric, actin-binding ATPase with a total molecular

weight of 480kD in a head-and-tail conformation. Myosin II has two identical head

domains, where the actin binding sites are localized. It consists of two heavy chains

(each 220kD) and four light chains (ELC=essential and RLC= regulatory light chains).

Actin interaction

Binds to F-actin

Form

Lyophilized, ready-to-use

Quantity

2 x 100 µg

Buffer

500mM KCl, 20mM Imidazole pH 7.0, 2mM DTT and 8% disaccharides,

when reconstituted with ultrapure water to obtain a 1mg/ml solution.

Purity

>95% by scanning densitometry from Coomassie G-250 stained SDS-Gels.

Purification notes

Purified from rabbit skeletal muscle (m. psoas).

Protein conc.

Determined at OD280 (0.1% = 0.53).

Storage instructions

Upon reconstitution the product is stored on ice. This product can also be stored

as a glycerol stock at -20°C. Avoid repeated freeze / thaw cycles.

Shipping conditions

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or

Therapeutical applications.

Protein

Myosin S1

Origin

Skeletal muscle, rabbit

Molecular mass

110kDa

Protein description

Myosin S1 obtained by chymotryptic cleavage of myosin II from rabbit psoas muscle

has a molecualr mass of 110kD. Myosin S1 is the motor domain of myosin II,

which contains the actin- and ATP-binding site.

Actin interaction

Binds to F-actin

Form

Lyophilized, ready-to-use

Quantity

1 x 500 µg

Buffer

50mM KCl, 10mM Tris pH 7.6, 0.2mM DTT and 1% disaccharides, when reconstituted

with 1ml ultrapure water.

Purity

>95% by scanning densitometry from Coomassie G-250 stained SDS-Gels.

Purification notes

Purified from rabbit skeletal muscle (m. psoas).

Protein conc.

Determined by Biuret method with actin (skeletal muscle, rabbit) as reference.

Storage instructions

Upon reconstitution the product is stored on ice. This product can also be stored

as a glycerol stock at -20°C. Avoid repeated freeze / thaw cycles.

Shipping conditions

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or

Therapeutical applications.

Protein

Myosin II

Origin

Skeletal muscle, rabbit (m.psoas)

Molecular mass

~480kDa

Protein description

Fast muscle myosin II from rabbit psoas muscle is a hexameric, actin-binding

ATPase with a total molecular weight of 480kD in a head-and-tail conformation.

Myosin II has two identical head domains, where the actin binding sites are

localized. It consists of two heavy chains (each 220kD) and four light chains

(ELC=essential and RLC= regulatory light chains).

Actin interaction

Binds to F-actin

Form

Lyophilized, ready-to-use

Quantity

4 x 100 µg

Buffer

500mM KCl, 20mM Imidazole pH 7.0, 2mM DTT and 8% disaccharides,

when reconstituted with 1.0ml ultrapure water to obtain a 1mg/ml solution.

Purity

>95% by scanning densitometry from Coomassie G-250 stained SDS-Gels.

Purification notes

Purified from rabbit skeletal muscle (m. psoas).

Protein conc.

Determined by Biuret method with actin (rabbit skeletal muscle) as reference.

Storage instructions

Upon reconstitution the product is stored on ice. This product can also be stored

as a glycerol stock at -20°C. Avoid repeated freeze / thaw cycles.

Shipping conditions

At ambient temperature. Upon delivery store at -70°C.

Remarks

For coated or filamentous myosin II MotorMix I (Cat. #: 5502-01) or

PolyMix (Cat. #: 5000-0) can be used. MotorMix II (Cat. #: 5503-01) is required for

soluble myosin II.