Products

 

 

G-Biosciences' Protein G Magnetic Beads are Fe₃O4 beads coated with dextran to produce highly uniform,

1µm beads. Recombinant Protein G is covalently coupled to the dextran coat to produce an enhanced

alternative to agarose slurries for immunoprecipitation experiments. G-Biosciences magnetic beads compare

well to leading magnetic beads (Figures 1 & 2).

 

 

Fig. 1 A comparison of Protein G Magnetic Beads. All beads were used as per the manufacturer's

instructions. Image highlights the low amounts of beads required from G-Biosciences and the tight packing

for easy handling.

 

 

Fig. 2  A comparison of immunoprecipitations of BSA with different magnetic Protein G beads. G-

Biosciences beads purify similar amounts of BSA and has low non specific protein binding comparable to

Life Technologies Dynabeads.

 

 

The use of magentic beads offers several distinct advantages of traditional immunoprecipitation experiments

include a significant reduction in time and non-specific protein binding.

 

A simple protocol involves the addition of your antibody of choice to the beads, which bind the Fc region

(See Table 1) during a short incubation.

 

Table 1  Relative affinity of Immobilized Protein A & Protein G for various antibody species & subclasses of IgGs.

 

 

The tube is placed on a magnet and the supernatant is removed by aspiration. The antibody bound magnetic

beads can be used in a variety of downstream processes including:

• Immunoprecipitations

• Co-immunoprecipitations

• Chromatin immunoprecipitation (ChIP)

• Small-scale IgG Purification and antibody labeling

• Protein Isolation

 

Features

• 1µm beads

• 260µg human IgG/ml

• Significant reduction in background due to non-specific binding

• Simple, short procedure

• No costly columns required

• No time consuming centrifugations required

• Gentle separation preserves native protein interactions

 

Applications

• Immunoprecipitations

• Co-immunoprecipitations

• Chromatin immunoprecipitation (ChIP)

• Small-scale IgG Purification and antibody labeling

• Protein Isolation