Products

Bacterial DNA Helicase ATPase Assays

DNA helicase (DnaB) hydrolyzes ATP as the source of molecular energy to carry out DNA unwinding required by

the DNA replication process.  Inhibition of the ATPase activity of DNA helicase blocks its DNA unwinding function.

The DNA helicase ATPase assay can be used for high-throughput screen of DNA helicase inhibitors in drug

discovery.  The DNA Helicase ATPase Assay Kit is based on detection of the phosphate produced by the ATP

hydrolysis reaction in the presence of DNA. The assay is in a 384-well plate format and the phosphate is detected

using light absorbance at 650 nm.

E. coli DNA Helicase ATPase assay Kit Plus-100

The E. coliDNA Helicase ATPase assay Kit Plus-100 includes 0.500 ml of 10 x assay buffer, 0.035 ml of 100 x DNA,

0.030 ml of 100 x E. coliDNA helicase, 0.035 ml of 100 x ATP and 5 ml of dye for 100 assays in a 384-well assay

format.

E. coli DNA Helicase ATPase assay Kit Plus-500

The E. coliDNA Helicase ATPase assay Kit Plus-500 includes 2 ml of 10 x assay buffer, 0.170 ml of 100 x DNA,

0.150 ml of 100 x E. coliDNA helicase, 0.170 ml of 100 x ATP and 25 ml of dye for 500 assays in a 384-well assay

format.

P. aeruginosa DNA Helicase ATPase assay kit Plus-100

The P. aeruginosaDNA Helicase ATPase assay Kit Plus-100 includes 0.500 ml of 10 x assay buffer, 0.035 ml of 100

x DNA, 0.030 ml of 100 x P. aeruginosaDNA helicase, 0.035 ml of 100 x ATP and 5 ml of dye for 100 assays in a

384-well assay format.

P. aeruginosa DNA Helicase ATPase assay kit Plus-500 (DNAB500KP)

The P. aeruginosaDNA Helicase ATPase assay Kit Plus-500 includes 2 ml of 10 x assay buffer, 0.170 ml of 100 x

DNA, 0.150 ml of 100 x P. aeruginosaDNA helicase, 0.170 ml of 100 x ATP and 25 ml of dye for 500 assays in a

384-well assay format.

H. influenzae DNA Helicase ATPase assay kit Plus-100

The H. influenzaeDNA Helicase ATPase assay Kit Plus-100 includes 0.500 ml of 10 x assay buffer, 0.035 ml of 100

x DNA, 0.030 ml of 100 x H. influenzaeDNA helicase, 0.035 ml of 100 x ATP and 5 ml of dye for 100 assays in a

384-well assay format.

H. influenzae DNA Helicase ATPase assay kit Plus-500

The H. influenzaeDNA Helicase ATPase assay Kit Plus-500 includes 2 ml of 10 x assay buffer, 0.170 ml of 100 x

DNA, 0.150 ml of 100 x H. influenzaeDNA helicase, 0.170 ml of 100 x ATP and 25 ml of dye for 500 assays in a 384-

well assay format.

S. pneumoniae DNA Helicase ATPase assay kit Plus-100

The S. pneumoniaeDNA Helicase ATPase assay Kit Plus-100 includes 0.500 ml of 10 x assay buffer, 0.035 ml of

100 x DNA, 0.030 ml of 100 x S. pneumoniaeDNA helicase, 0.035 ml of 100 x ATP and 5 ml of dye for 100 assays in

a 384-well assay format.

S. pneumoniae DNA Helicase ATPase assay kit Plus-500

The S. pneumoniaeDNA Helicase ATPase assay Kit Plus-500 includes 2 ml of 10 x assay buffer, 0.170 ml of 100 x

DNA, 0.150 ml of 100 x S. pneumoniaeDNA helicase, 0.170 ml of 100 x ATP and 25 ml of dye for 500 assays in a

384-well assay format.

S. aureus DNA Helicase ATPase assay Kit Plus-100

The S. aureusDNA Helicase ATPase assay Kit Plus-100 includes 0.500 ml of 10 x assay buffer, 0.035 ml of 100 x

DNA, 0.030 ml of 100 x S. aureusDNA helicase, 0.035 ml of 100 x ATP and 5 ml of dye for 100 assays in a 384-well

assay format.

S. aureus DNA Helicase ATPase assay Kit Plus-500

The S. aureusDNA Helicase ATPase assay Kit Plus-500 includes 2 ml of 10 x assay buffer, 0.170 ml of 100 x DNA,

0.150 ml of 100 x S. aureusDNA helicase, 0.170 ml of 100 x ATP and 25 ml of dye for 500 assays in a 384-well

assay format.

Catalog No.

Product Name

Size

DNAB100KE

E. coli DNA Helicase ATPase assay Kit Plus-100

100 assays

DNAB500KE

E. coli DNA Helicase ATPase assay Kit Plus-500

500 assays

DNAB100KP

P. aeruginosa DNA Helicase ATPase assay kit Plus-100

100 assays

DNAB500KP

P. aeruginosa DNA Helicase ATPase assay kit Plus-500

500 assays

DNAB100KH

H. influenzae DNA Helicase ATPase assay kit Plus-100

100 assays

DNAB500KH

H. influenzae DNA Helicase ATPase assay kit Plus-500

500 assays

DNAB100KN

S. pneumoniae DNA Helicase ATPase assay kit Plus-100

100 assays

DNAB500KN

S. pneumoniae DNA Helicase ATPase assay kit Plus-500

500 assays

DNAB100KS

S. aureus DNA Helicase ATPase assay Kit Plus-100

100 assays

DNAB500KS

S. aureus DNA Helicase ATPase assay Kit Plus-500

500 assays