Products

 

 

G-Biosciences' Protein A Magnetic Beads are Fe₃O4 beads coated with dextran to produce highly uniform,

1µm beads. Recombinant Protein A is covalently coupled to the dextran coat to produce an enhanced

alternative to agarose slurries for immunoprecipitation experiments.

The use of magentic beads offers several distinct advantages of traditional immunoprecipitation experiments

include a significant reduction in time and non-specific protein binding.

A simple protocol involves the addition of your antibody of choice to the beads, which bind the Fc region

(See Table 1) during a short incubation.

 

Table 1 Relative affinity of Immobilized Protein A & Protein G for various antibody species & subclasses of IgGs.

 

 

Fig. 1 Protein A and G Magnetic Bead Immunoprecipitation of NFκB

 

Fig. 2 Purification of immunoglobulins with Protein A magnetic beads

 

 

The tube is placed on a magnet and the supernatant is removed by aspiration. The antibody bound magnetic

beads can be used in a variety of downstream processes including:

• Immunoprecipitations (Figure 1)

• Co-immunoprecipitations (Figure 3)

• Chromatin immunoprecipitation (ChIP)

• Small-scale IgG Purification and antibody labeling (Figure 2)

• Protein Isolation

 

 

Fig. 3 Coimmunoprecipitation of SUV3 with PNP using Protein A Magnetic Beads

 

 

Features

• 1µm beads
• 260µg human IgG/ml
• Significant reduction in background due to non-specific binding
• Simple, short procedure
• No costly columns required
• No time consuming centrifugations required
• Gentle separation preserves native protein interactions

 

Applications

• Immunoprecipitations
• Co-immunoprecipitations
• Chromatin immunoprecipitation (ChIP)
• Small-scale IgG Purification and antibody labeling
• Protein Isolation