Products

 

AlamarBlue Cell Viability Reagent quantitatively measures the proliferation of mammalian cell lines, bacteria

and fungi. The dye incorporates an oxidation-reduction (REDOX) indicator that both fluoresces and change

color in response to the chemical reduction due to cell growth. Since, the AlamarBlue is very stable and

nontoxic to the cells, continuous monitoring of cultures over time is possible.

 

The alamarBlue dye in its oxidized form is blue in color and non-fluorescent. In alamarBlue Reagent assay,

the growing cells cause a chemical reduction of the alamarBlue dye from non-fluorescent blue to fluorescent

red. The continued growth of viable cells maintain a reducing environment (fluorescent, red) and inhibition of

growth maintains an oxidized environment (non-fluorescent, blue), which can be detected using a

fluorescence or absorbance detector.

 

 

       Conversion of alamarBlue dye from oxidized to reduced form