Products

CytoScan™ - WST-1 Cell Proliferation Assay

CytoScan™ WST-1 Cell Proliferation Assay is a sensitive and accurate assay for cell proliferation and

cytotoxicity.  The assay is highly convenient as it is performed in a single cell tissue culture well and

requires no washing, harvesting or solubilization of cells.

Adherent or suspension cells are cultured in a microplate and then incubated with WST-1 and the assay is

monitored with a spectrophotometer. The assay principle is based upon the reduction of the tetrazolium salt

WST-1 to formazan by cellular dehydrogenases (see figure below).  The generation of the dark yellow

colored formazan is measured at 420-480nm (optimal at 440nm) and is directly correlated to cell number.

Features

• Uses WST-1, a high sensitivity tetrazolium salt.

• Colorimetric assay.

• Adherent or suspension cells.

• No washing, harvesting or solubilization required.

Applications

• For the detection of cell toxicity, death, viability or proliferation.

• Ideal for high throughout screening

References

• Hsieh, C. et al (2014) Food Funct. 5: 2494

• Schweigmann, H. et al (2014) J. Steroid Biochem. Mol. Biol. 143:259

• Galas, R.J. and Liu, J. (2014) J. Cell. Biochem. 115:111

• Kuo, C. et al (2013) Allergy. 68:870

• Chen, Y. et al (2013) Langmuir. 29:3721

• Hagmann, W. et al (2011) Cancers. 3:106

• Neelofar, K. et al (2011) Can. J. Microbiol. 57:204

• Li, H. et al (2009) Mol Cancer Ther 8:3255

Instruction sheet(PDF)

Catalog No.

Product Name

Size

786-212

              CytoScan™ - WST-1 Cell Proliferation Assay

500 assays

786-857

              CytoScan™ - WST-1 Cell Proliferation Assay

       2,000 assays