Products

Bacterial Kinase assay kits

Guanylate Kinase assay kit

Guanylate kinase is essential for recycling GMP and cGMP.  It catalyzes the ATP-dependent

phosphorylation of GMP into GDP.  Guanylate kinase is an attractive antibacterial target.  The guanylate

                           GMP + ATP =  GDP + ADP

kinase assay is based on measurement of ADP generated from the kinase reaction.  The assay is

fluorescence-based and can be carried out using regular black 96-well or 384-well plates or cuvettes.

The E. coli Guanylate Kinase Assay Kit Plus (Catalog No.  GMK100KE) includes 600 µl of 10 x assay buffer,

35 µl of 100 x GMP, 35 µl of 100 x E. coliGMP kinase, 35 µl of 100 x ATP, 35 µl of 100 x MUK Reagent A, 35 µl

of 100 x MUK Reagent B and 350 µl of 10 x fluorescence dye for 100 assays in a 384-well assay format.

The E. coli Guanylate Kinase Assay Kit Plus-500 (Catalog No.  GMK500KE) includes 2000 µl of 10 x assay

buffer, 170 µl of 100 x GMP, 170 µl of 100 x E. coliGMP kinase, 170 µl of 100 x ATP, 170 µl of 100 x MUK

Reagent A, 170 µl of 100 x MUK Reagent B and 1700 µl of 10 x fluorescence dye for 500 assays in a 384-well

assay format.

The S. pneumoniae GMP Kinase Assay Kit Plus-500 (Catalog No.  GMK500KN) includes 2000 µl of 10 x

assay buffer, 170 µl of 100 x GMP, 170 µl of 100 x S. pneumoniae GMP kinase, 170 µl of 100 x ATP, 170 µl of

100 x MUK Reagent A, 170 µl of 100 x MUK Reagent B and 1700 µl of 10 x fluorescence dye for 500 assays in

a 384-well assay format.

Bacterial UMP Kinase assay kit

UMP kinase or uridylate kinase is essential for biosynthesis of nucleic acid. It catalyzes the ATP-dependent

phosphorylation of UMP into UDP.

                           UMP + ATP = UDP + ADP

UMP kinase is an attractive antibacterial target.  The UMP kinase assay is based on measurement of ADP

generated from the kinase reaction.  The assay is fluorescence-based and can be carried out using regular

black 96-well or 384-well plates or micro-cuvettes.  

The E. coli UMP Kinase Assay Kit Plus (Catalog No.  UMK100KE) includes 600 µl of 10 x assay buffer, 35 µl

of 100 x UMP, 35 µl of 100 x E. coli UMP kinase, 35 µl of 100 x ATP, 35 µl of 100 x MUK Reagent A, 35 µl of 100

x MUK Reagent B and 300 µl of 10 x fluorescence dye for 100 assays in a 384-well assay format.

The E. coli UMP Kinase Assay Kit Plus-500 (Catalog No.  UMK500KE) includes 2 ml of 10 x assay buffer,

170 µl of 100 x UMP, 170 µl of 100 x E. coli UMP kinase, 170 µl of 100 x ATP, 170 µl of 100 x MUK Reagent A,

170 µl of 100 x MUK Reagent B and 1.7 ml of 10 x fluorescence dye for 500 assays in a 384-well assay

format.

Bacterial NAD+ Kinase assay kit

NAD⁺kinaseconverts NAD⁺into NADP⁺by phosphorylation in the presence of ATP.  NADP⁺plays key roles in

energy transduction and various biochemical process including DNA repair, protein modification and cell

signaling.  

                            NAD⁺  + ATP = NADP⁺ + ADP

NAD⁺kinaseis an attractive antibacterial target.  The NAD⁺kinaseassay is based on measurement of ADP

generated from the kinase reaction.  The assay is fluorescence-based and can be carried out using regular

black 96-well or 384-well plates or micro-cuvettes.

The E. coliNAD+Kinase Assay Kit Plus (Catalog No.  NADK100KE) includes 600 µl of 10 x assay buffer, 35 µl

of 100 x NAD+, 35 µl of 100 x E. coliNAD kinase, 35 µl of 100 x ATP, 35 µl of 100 x MUK Reagent A, 35 µl of

100 x MUK Reagent B and 300 µl of 10 x fluorescence dye for 100 assays in a 384-well assay format.

The E. coli NAD+Kinase Assay Kit Plus-500 (Catalog No.  NADK 500KE) includes 2 ml of 10 x assay buffer,

170 µl of 100 x NAD+, 170 µl of 100 x E. coli NAD kinase, 170 µl of 100 x ATP, 170 µl of 100 x MUK Reagent A,

170 µl of 100 x MUK Reagent B and 1.7 ml of 10 x fluorescence dye for 500 assays in a 384-well assay

format.