Products

Thymidylate Kinase assay kits

Human Thymidylate Kinase assay kit

Thymidylate kinase is an important enzyme in the dTTP synthesis pathway for DNA synthesis. The

thymidylate kinase activity is higher in the tumor than in the corresponding normal human tissue.  The

function of this enzyme is to catalyze the phosphorylation of dTMP using ATP to form dTDP and ADP.  The

thymidylate kinase assay is based on measurement of ADP generated from the kinase reaction.  The assay

is fluorescence-based and can be carried out using regular black or white 96-well or 384-well plates or

micro-cuvettes.  

The Human Thymidylate Kinase Assay Kit Plus-500 (Catalog No.  HTMK500KE) includes 1700 µl of 10 x

reaction buffer, 8 µl of 2500 x human thymidylate kinase (125 mM), 170 µl of 100 x ATP (5 mM), 170 µl of 100 x

dTMP (40 mM), 170 µl of 100 x MUK Reagent A, 170 µl of 100 x MUK Reagent B and 1700 µl of 10 x

fluorescence dye.  The kit reagents are sufficient for 500 thymidylate kinase assays using a standard black

384-well plate (Matrix 4318).  

Bacterial Thymidylate Kinase assay kit

Thymidylate kinase is an important enzyme in the dTTP synthesis pathway for DNA synthesis. The function

of this enzyme is to catalyze the phosphorylation of dTMP using ATP to form dTDP and ADP.

                           dTMP + ATP = dTDP + ADP

Thymidylate kinase is an attractive antibacterial target.  The thymidylate kinase assay is based on

measurement of ADP generated from the kinase reaction.  The assay is fluorescence-based and can be

carried out using regular black 96-well or 384-well plates or micro-cuvettes.

The E. coli Thymidylate Kinase Assay Kit Plus (Catalog No. TMK100KE) includes 600 µl of 10 x Buffer, 35 µl

of 100 x dTMP, 35 µl of 100 x E. coli Thymidylate kinase, 35 µl of 100 x ATP, 35 µl of 100 x MUK Reagent A,

35 µl of 100 x MUK Reagent B and 300 µl of 10 x fluorescence dye for 100 assays in a 384-well assay format.

The kit contains the 10 x reaction buffer (10 x MUK buffer), E. coli thymidylate Kinase, dTMK substrate, 100 x

MUK reagent 1, 100 x MUK reagent 2 and 10 x MUK dye. The kit reagents are sufficient for 500 assays of E.

coli thymidylate kinase using a standard black or white 384-well plate.