Products

MicroMolar Universal Kinase assay kits

The MicroMolar Universal Kinase Assay Kit is based on measurement of ADP generated from the kinase

reaction.  It is used to measure the activities of purified kinases producing ADP at concentrations ranging

from 0.1 mM to 10 mM.

                           Substrate + ATP = Phosphorylated substrate + ADP

The assay is fluorescence-based and can be carried out using regular black or white 96-well or 384-well

plates or micro-cuvettes.  It is important to avoid using unnecessarily high ATP concentrations in the kinase

assays for the following reasons.  One is to allow the assay to detect ATP-competitive inhibitors.  The other

is to avoid a high ADP background from ADP contamination in the ATP samples.  It is recommended to use

ATP concentrations at 20 µM (micromolar).

The MicroMolar Universal Kinase Assay Kit   (Catalog No.  MUK100K) includes 600 µl of 10 x assay buffer,

35 µl of 100 x ATP, 35 µl of 100 x MUK Reagent A, 35 µl of 100 x MUK Reagent B, 30 µl of 1 mM ADP and

350 µl of 10 x fluorescence dye for 100 assays in a 384-well assay format.  

The MicroMolar Universal Kinase Assay Kit-1000   (Catalog No.  MUK1000K) includes 4000 µl of 10 x assay

buffer, 350 µl of 100 x ATP, 350 µl of 100 x MUK Reagent A, 350 µl of 100 x MUK Reagent B, 200 µl of 1 mM

ADP and 3500 µl of 10 x fluorescence dye for 1000 assays in a 384-well assay format.