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Mass spectrometry has become a popular method for analysis and characterization of proteins. Traditional

total protein extraction method such as RIPA buffer-based protein extraction suffers the disadvantages of

incomplete protein extraction. Some components in RIPA buffer may not compatible with certain downstream

applications. MinuteTM total protein extraction kit for mass spectrometry is specifically developed to

overcome these short comings. This kit can extract total protein from animalcultured cells/tissues efficiently

and rapidly without bias. The extracted proteins are compatible with trypsin digestion, iTRAQ labeling and

biotin-labeling. Due to the use of the protein extraction filter cartridges, the extraction volume can be as small

as 20 µl and as large as 500 µl. This unique feature is very useful in situations where available starting

material is a limiting factor. Total proteins can be extracted rapidly with high yield (2-8 mg/ml).

Comparison between a rapid (1- 3 min) novel protein extraction kit and RIPA buffer (PDF)