Products

Reverse transcriptase assay kits

M-MLV(Moloney murine leukemia virus) reverse transcriptase assay

The Moloney murine leukemia virus (M-MLV) causes lymphocytic leukemia in mice. M-MLV reverse

transcriptase (M-MLV RT) synthesizes a complementary DNA strand from single-stranded RNA or DNA.

The M-MLV Reverse Transcriptase Assay is based on measurement of the DNA molecules synthesized by

M-MLV RT.  The assay can be performed in a 384-well or 96-well plate format for tests of M-MLV reverse

transcriptase activities and high throughput screening of inhibitors.

The M-MLV Reverse Transcriptase Assay Kit (Catalog number: MLV100K) includes 400 µl of 10 x Buffer,

33 µl of 100 x Template, 33 µl of 100 x dNTPs, 1550 µl of 2 x Dye and 1550 µl of 50 mM EDTA. It is for 100

assays of M-MLV Reverse Transcriptase. The assay kit includes all reagents except the enzyme.   

The M-MLV Reverse Transcriptase Assay Plus(Catalog number: MLV100KE) includes all reagents in the M-

MLV Reverse Transcriptase Assay Kit (Catalog number: MLV100K) plus the enzyme, 7 µl 500 x M-MLV RT.

HIV reverse transcriptase

Infection of human immunodeficiency virus (HIV) causes acquired immunodeficiency syndrome (AIDS). The

HIV reverse transcriptase (HIV RT) is an attractive drug target for HIV drug discovery. The HIV RT enzyme

synthesizes a complementary DNA strand initiating from a primer using either RNA or single-stranded DNA

as a template. The HIV Reverse Transcriptase Assay is based on measurement of the DNA molecules

synthesized by HIV RT.  The assay can be performed in a 384-well or 96-well plate format for tests of HIV

reverse transcriptase activities and high throughput screening of inhibitors.

The HIV Reverse Transcriptase Assay Kit (Catalog number: HIV100K) includes 400 µl of 10 x Buffer, 33 µl of

100 x Template, 33 µl of 100 x dNTPs, 1550 µl of 2 x Dye and 1550 µl of 50 mM EDTA. It is for 100 assays of

HIV Reverse Transcriptase. The assay kit includes all reagents except the enzyme.   

The HIV Reverse Transcriptase Assay Plus(Catalog number: HIV100KE) includes all reagents in the HIV

Reverse Transcriptase Assay Kit (Catalog number: HIV100K) plus the enzyme, 7 µl 500 x HIV RT.

AMV(Avian myeloblastosis virus) reverse transcriptase assay kit

The avian myeloblastosis virus (AMV) is an alpha retrovirus responsible for acute myeloblastic leukemia

(AML). AMV Reverse Transcriptase (AMV RT) catalyzes the synthesis of DNA using RNA or DNA template

and dNTPs. The AMV Reverse Transcriptase Assay is based on measurement of the DNA molecules

synthesized by AMV RT.  The assay can be performed in a 384-well or 96-well plate format for tests of AMV

reverse transcriptase activities and high throughput screening of inhibitors.

The AMV Reverse Transcriptase Assay Kit (Catalog number: AMV100K) includes 400 µl of 10 x Buffer, 33 µl

of 100 x Template, 33 µl of 100 x dNTPs, 1550 µl of 2 x Dye and 1550 µl of 50 mM EDTA. It is for 100 assays of

AMV Reverse Transcriptase. The assay kit includes all reagents except the enzyme.   

The AMV Reverse Transcriptase Assay Plus(Catalog number: AMV100KE) includes all reagents in the AMV

Reverse Transcriptase Assay Kit (Catalog number: AMV100K) plus the enzyme, 7 µl 500 x AMV RT.