Products

96-Well DNA Decatenation Assays

Human DNA topoisomerase II and bacterial DNA topoisomerase IV (the parC-parE complex) converts the

concatenated DNA into decatenated DNA (decatenation reaction).   Bacterial topoisomerase I and gyrases

also have DNA decatenation activity.  The 96-Well Topoisomerase DNA Decatenation Assays are in a 96-well

assay plate format that can be used for high-throughput tests of human topo II and bacterial topo IV inhibitors.

The assay is based on the principle that the decatenated DNA is separated from the concatenated DNA by a

filtration process.  The decatenated DNA passed through the filter (TDD filter plate), received in a 96 well plate

and quantified by fluorescence at 535 nm (excitation at 485 nm).

96-Well Topoisomerase DNA Decatenation Assay Kit

Includes 600 µl of 10 x assay buffer, 500 µl of 10 x concatenated DNA, 110 µl of 10 mM ATP, 600 µl of 0.4 M

EDTA, 260 µl of 20 x fluorescence dye, 2 ml of 10 x rinse buffer, one V-bottom plate, a TDD filter plate and

one black 96-well plates for 96 assays of DNA decatenation reactions. The assay buffer is optimized for

bacterial topoisomerase IV.  DNA decatenation enzyme is not included. This kit can be used for DNA

decatenation assays of any bacterial topoisomerase IV (the parC-parE complex) enzymes or gyrases.

96-Well Human Topo II DNA Decatenation Assay Kit Plus

Includes the assay buffer, concatenated DNA, human topo II alpha, ATP, fluorescence dye, rinse buffer and

a TDD filter plate for 96 assays of human topo II DNA decatenation reactions.

96-Well E. coli Topo IV DNA Decatenation Assay Kit Plus

Includes 600 µl of 10 x assay buffer, 500 µl of 10 x concatenated DNA, 110 µl of 10 mM ATP, 8 µl of 1000 x E.

colitopo IV, 600 µl of 0.4 M EDTA, 260 µl of 20 x fluorescence dye, 2 ml of 10 x rinse buffer, one V-bottom

plate, a TDD filter plate and one black 96-well plates for 96 assays of DNA decatenation reactions.

96-Well S. aureus Topo IV DNA Decatenation Assay Kit Plus

Includes 600 µl of 10 x assay buffer, 500 µl of 10 x concatenated DNA, 110 µl of 10 mM ATP, 8 µl of 1000 x S.

aureustopo IV, 600 µl of 0.4 M EDTA, 260 µl of 20 x fluorescence dye, 2 ml of 10 x rinse buffer, one V-bottom

plate, a TDD filter plate and one black 96-well plates for 96 assays of DNA decatenation reactions.

96-Well S. pneumoniae Topo IV DNA Decatenation Assay Kit Plus

Includes 600 µl of 10 x assay buffer, 500 µl of 10 x concatenated DNA, 110 µl of 10 mM ATP, 8 µl of 1000 x S.

pneumoniae topo IV, 600 µl of 0.4 M EDTA, 260 µl of 20 x fluorescence dye, 2 ml of 10 x rinse buffer, one V-

bottom plate, a TDD filter plate and one black 96-well plates for 96 assays of DNA decatenation reactions.

96-Well P. aeruginosa Topo IV DNA Decatenation Assay Kit Plus

Includes 600 µl of 10 x assay buffer, 500 µl of 10 x concatenated DNA, 110 µl of 10 mM ATP, 8 µl of 1000 x P.

aeruginosa topo IV, 600 µl of 0.4 M EDTA, 260 µl of 20 x fluorescence dye, 2 ml of 10 x rinse buffer, one V-

bottom plate, a TDD filter plate and one black 96-well plates for 96 assays of DNA decatenation reactions.

96-Well E. coli Topo I DNA Decatenation Assay Kit Plus

Includes the assay buffer, concatenated DNA, E. coli topo I, fluorescence dye, rinse buffer and a TDD filter

plate for 96 assays of E. coli topo I DNA decatenation reactions.  

96-Well E. coli gyrase DNA Decatenation Assay Kit Plus

Includes the assay buffer, concatenated DNA, E. coli gyrase, ATP, fluorescence dye, rinse buffer and a TDD

filter plate for 96 assays of E. coli gyrase DNA decatenation reactions.  

96-Well S. aureus gyrase DNA Decatenation Assay Kit Plus

Includes 600 µl of 10 x assay buffer, 500 µl of 10 x concatenated DNA, 120 µl of 10 mM ATP, 100 µl of 50 x S.

aureusgyrase, 600 µl of Stop solution (0.4 M EDTA), 500 µl of 10 x fluorescence dye, 2 ml of 10 x rinse buffer,

one V-bottom plate, a TDD filter plate and one black 96-well plates for 96 assays of DNA decatenation

reactions.