Products

Preparation of cytosolic and nuclear fractions from cultured cells and tissues is a common practice. Cell

fractionation from cultured cells is relatively easy and straight forward. However, a clear separation of

cytosolic and nuclear fractions from tissues, especially frozen tissues, is challenging. The cross-

contaminations from frozen tissues are always troublesome due to the altered tissue cellular structurecaused

by freeze and thaw cycle, improper homogenization and inadequate extraction buffer used. Majority of

current commercial kits are designed to treat cultured cells and tissues similarly without paying sufficient

attention to the structure difference between the two types of samples. As a result, cross-contamination of

cytosolic and nuclear fractions from fresh/ frozen tissues remains as a major problem (see references 1 and

2 below). This novel cytosolic and nuclear extraction kit is specially designed toaddress the issue by

employing proprietary buffers and unique protocol to minimize the cross-contaminations for fresh and frozen

tissue samples.