Products

Endoplasmic reticulum (ER) is a major membranous structure that functionally connects nuclear membrane

and plasma membrane. ER plays a central role in exocytic pathway of protein trafficking in all eukaryotic

cells. Proteins synthesized in cytoplasm are translocated into ER from where vesicles shuttle protein cargo

to the Golgi apparatus, which subsequently fuses with plasma membrane. Traditional methods for isolating

ER are based on density-gradient ultracentrifugation.The protocol requires a large amount of starting material

and the methods are tedious and time-consuming, and contain significant cross-contamination. Currently, all

commercial kits for ER isolation are based on methods developed in the 1970s. Unlike any other ER isolation

kit on the market, our ER isolation kit employs a patented spin-column-based technology that is simple and

rapid and requires only a small amount of starting cultured cells or tissues. This kit can differentially

precipitate native ER (mainlyrough ER) from cultured cells/tissues without using a Dounce homogenizer or

ultracentrifugation. The whole protocol can be done in about 2 hours.

Enrichment of ER from mouse tissue.  A. Ponceau S stained blot.  B. Western blot of ER, plasma membrance

and cytosolic markers.  ER-hCalreticulin (R&D System, MAB38981). Na/K ATPase (Novus, NB3001 46SS);

GAPDH (Biolegend, 631401).  1. Total tissue lysate, 2 and 3. Extracted ER fraction in duplicate.