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A tendon/ligament is a fibrous connective tissue that attaches muscles to bone. Histologically tendons

consist of dense regular collagen fibers that are very tough and notoriously difficult to homogenize. It is also

very difficult to lyse the tendon tissues for total protein extraction. Traditional solution-based protein extraction

methods, such as RIPA, are inefficient and the protein yield is very low. Theprofile of extracted protein is also

biased (incomplete protein profile) with solution-based methods. This kit provides a highly efficient method

for total protein extraction from human or animal tendons by a combination of mechanical extraction and

chemical lysis. The cell lysis buffers used are much stronger than RIPA buffers. The kit features a simple,

fast single-tube protocol and optimized buffers for tendons. Researchers have the option to choose either a

denaturing or native cell lysis buffer, which isspecifically tailored for tendons. The whole procedure takes

less than 10 minutes to complete and the protein yield is in the range of 1-3 mg/ml. The materials provided

are sufficient for 50 extractions.

Comparison of protein extraction efficiency from port artery

(RIPA vs SA-04-TD)

Same amount of staring tissue and same volume of lysis buffer (200ul) were used. For RIPA buffer the tissue

was homogenized in a 1.5ml tube with a pestle for 5 minutes and incubated at RT for 10 minutes. The

homogenate was centrifuged at 10,000 x g for 10 minutes and supernatant saved. Same volume of extracted

protein was loaded in 10% SDS-PAGE and stained with Coommassie blue. For SA-04-TD denaturing

bufferwasused. Lane 1. RIPA buffer (R0278, Sigma). Lane 2. SA-04-TD. 

Shipping and storage: This kit is shipped and stored at room temperature.