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Blood vessels (arteries and veins) are made of specialized connective tissues. There are three layers of

tissues in blood vessels: tunica, tunica media and endothelium. Due to their unique structures, blood vessels

are notoriously difficult to homogenize. It is also very difficult to lyse the cells in the tissue for total protein

extraction. Traditional solution-based protein extraction methods, such as RIPA,are inefficient and the protein

yield is very low. The profile of extracted protein is also biased with solution-based (incomplete protein

profile) methods. This kit provides a highly efficient method for total protein extraction from human or animal

blood vessels by a combination of mechanical extraction and chemical lysis. The cell lysis buffers used are

much stronger than the RIPA buffer. The kit features a simple, fast single-tube protocol and optimized buffers

for blood vessels. Researchers have the optionto choose either a denaturing or native cell lysis buffer, which

is specifically tailored for blood vessels. The whole procedure takes less than 10 minutes to complete and

the protein yield is in the range of 1-3 mg/ml. The materials provided are sufficient for 50 extractions.

Comparison of protein extraction efficiency from port artery

(RIPA vs SA-03-BV)

Same amount of staring tissue and same volume of lysis buffer (200ul) were used. For RIPA buffer the tissue

was homogenized in a 1.5ml tube with a pestle for 5 minutes and incubated at RT for 10 minutes. The

homogenate was centrifuged at 10,000 x g for 10 minutes and supernatant saved. Same volume of extracted

protein was loaded in 10% SDS-PAGE and stained with Coommassie blue. For SA-03-BV denaturing

bufferwas used. Lane 1. RIPA buffer (R0278, Sigma). Lane 2. SA-03-BV.

Shipping and storage: This kit is shipped and stored at room temperature.