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Bone tissues are commonly used for research. There are two types of bone tissues: compact and spongy.

Cells in bone tissues are tightly packed and, because of their unique structures, it is very difficult to efficiently

extract total protein from them. Traditional solution-based protein extraction methods, such as RIPA, are

inefficient and the protein yield is very low. The profile of extracted protein is also incomplete with solution-

based methods. This kit provides a highly efficient methodfor extracting proteins from human or animal bone

tissue by a combination of mechanical extraction and chemical lysis of cells in tissues. The kit features a

simple and fast single-tube protocol and optimized buffers for bone tissue. Researchers have the option to

choose either a denaturing or native cell lysis buffer, which is specifically tailored for bone tissue. The whole

procedure takes less than 10 minutes to complete and the protein yield is in the range of 0.5-2 mg/ml

depending upon type of bones. Thematerials provided are sufficient for 50 extractions.

Comparison of protein extraction efficiency from mouse tibia

(RIPA vs SA-02-BT)

Same amount of staring tissue and same volume of lysis buffer (200ul) were used. For RIPA buffer the tissue

was homogenized in a 1.5ml tube with a pestle for 5 minutes and incubated at RT for 10 minutes. The

homogenate was centrifuged at 10,000 x g for 10 minutes and supernatant saved. Same volume of extracted

protein was loaded in 10% SDS-PAGE and stained with Coommassie blue. For SA-02-BT denaturing

bufferwas used. Lane 1. RIPA buffer (R0278, Sigma). Lane 2. SA-02-BT.

Shipping and storage: This kit is shipped and stored at room temperature.