Products

Nucleoplasm, also referred to as karyoplasm/nucleus sap, consists of chromosomes, nucleolus and

nuclear matrix. Nucleoplasm plays a central role in genetic information flow and regulation of gene

expression. Nuclear protein isolation has traditionally been accomplished by extraction protocol using

detergent-containing buffers, which is effective in extracting total nuclear proteins but unable to separate

membrane-boundproteins and water-soluble matrix proteins. The detergent-free nuclear matrix protein

extraction kit is designed to separate cellular protein into three fractions: cytosolic, nuclear matrix and

water-insoluble fractions(mainly envelope and nucleic acid-associated proteins) using a patented spin-

column-based technology. The buffers used are detergent and EDTA-free. The whole protocol can be

completed in less than 1 hour. The proteins extracted are in their native state and can be used in

manydownstream applications such as gel retardation, transcription factor analysis, apoptosis and protein

trafficking studies.  

Western blotting of nuclear matrix protein. A. Ponceau S stain of transferred blot. TL, total cell lysate; WI,

water-insoluble fraction; MA, matrix protein. B. Western blot, anti-nuclear matrix marker p84 (GeneTex, DTX

70220).

Western blot of frozen mouse kidney fractions, TK, total kidney lysate; WIF, water-insoluble fraction; MA,

matrix protein. P84 (GeneTex, GTX70220), a matrix protein marker. (Lamin B1, Boster PB9611), a nuclear

envelope marker.

A. Human cell (HGPS), B. Mouse fibroblast. Extracted proteins were detected by Western blotting. C, Control

cells, T, Treated cells.