Hypermol EK
 
작성일 : 14-09-11
[Hypermol EK] Atto-Actin for TIRFM, Fluorescent Actin-Conjugates
Atto565-Actin / Atto488-Actin / Atto550-Actin / Atto647-Actin

 

Atto-Actin for TIRFM

 

Fluorescent Actin-Conjugates

Ready-for-TIRFM

 

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Highly photostable Atto-Actins are ideal to visualize actin dynamics by TIRFM.

 

Atto-fluorescent actin conjugates for TIRFM are labelled with a DOL of 0.3 and thus ready-for TIRFM.

The novel Atto-Actins are characterized by high photo stability, strong absoprtion and high fluorescence

quantum yields - some of several features demanded for todays imaging techniques.

 

These fluorescent actin conjugates are prepared by esterification of lysine residues with activated

Atto dyes. Atto-Actins are validated to possess the polymerization properties of native actin.

 

Atto-Actins are worldwide exclusively manufactured by Hypermol.

 

Applications

TIRFM, STED, GSD.

 

 

Atto565-Actin for TIRFM (alpha skeletal muscle actin, rabbit)

 

Description

Protein

 

Atto565-Actin

Origin

 

skeletal muscle alpha actin, rabbit

Molecular mass

 

42kDa

Protein description

 

 

 

 

 

Actin from rabbit skeletal muscle is a single chain polypeptide with a molecular

weight of 42kD consisting of 375 amino acids. Atto565-Actin is chemically modified

G-actin with an Atto565 fluorescent group covalently bound to lysine residues.

Atto565-Actin (λem 563nm, λem 592nm) possesses the polymerization properties

of native G-actin.

Conjugation ratio

 

 

 

This product is supplied with a degree of labelling (DOL) between 0.3

The precise DOL is stated in the DataSheet supplied with the product.

 

Properties

Form

 

Lyophilized, ready-to-use

Quantity

 

1x100 µg, 5x100 µg

 

Buffer

 

 

 

 

 

2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3%

disaccharides, when reconstituted with 1.0 ml ultrapure water to obtain a 1mg/ml

solution.

 

Purity

 

 

>99% by scanning densitometry

 

Purification notes

 

 

Purified from rabbit skeletal muscle.

 

Protein conc.

 

 

Determined by the Biuret method.

 

Storage instructions

 

 

 

Atto565-Actins are stored at -70°C upon arrival will be stable in performance for

at least 6 months from the date of purchase. The Solubilized product is kept on ice,

protect from light and it should be used within 1 week. Avoid refreezing.

 

Shipping conditions

 

 

At ambient temperature. Upon delivery store at -70°C.

 

Remarks

 

 

 

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or

Therapeutical Applications.

 

 

Atto488-Actin for TIRFM (alpha skeletal muscle actin, rabbit)

 

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ATTO 488

 

High fluorescence yield

• Strong absorption & high photo-stability

 

 

Description

Protein

 

Atto488-Actin

Origin

 

skeletal muscle alpha actin, rabbit

Molecular mass

 

42kDa

Protein description

 

 

 

 

 

Actin from rabbit skeletal muscle is a single chain polypeptide with a molecular

weight of 42kD consisting of 375 amino acids. Atto488-Actin is chemically modified

G-actin with an Atto488 fluorescent group covalently bound to lysine residues.

Atto488-Actin (λem 488nm, λem 523nm) possesses the polymerization properties

of native G-actin.

Conjugation ratio

 

 

 

This product is supplied with a degree of labelling (DOL) of ~0. 3.

The precise DOL is stated in the DataSheet supplied with the product.

 

Properties

Form

 

Lyophilized, ready-to-use

Quantity

 

1x100 µg, 5x100 µg

Buffer

 

 

 

 

2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3%

disaccharides, when reconstituted with 1.0 ml ultrapure water to obtain a 1mg/ml

solution.

 

Purity

 

 

>99% by scanning densitometry

 

Purification notes

 

 

Purified from rabbit skeletal muscle, GPC.

 

Protein conc.

 

 

Determined by the Biuret method.

 

Storage instructions

 

 

 

Atto565-Actins are stored at -70°C upon arrival will be stable in performance for

at least 6 months from the date of purchase. Solubilized Atto488-Actin is kept

on ice, protected from light and should be used within 1 week. Avoid refreezing.

 

Shipping conditions

 

 

At ambient temperature. Upon delivery store at -70°C.

 

Remarks

 

 

 

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or

Therapeutical Applications.

 

 

Atto550-Actin for TIRFM (alpha skeletal muscle actin, rabbit)

클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

ATTO 550

 

High fluorescence yield

• Strong absorption, high photo-stability

• High resolution microscopy, single molecule detection

 

Description

Protein

 

Atto550-Actin

Origin

 

skeletal muscle alpha actin, rabbit

Molecular mass

 

42kDa

Protein description

 

 

 

 

 

Actin from rabbit skeletal muscle is a single chain polypeptide with a molecular

weight of 42kD consisting of 375 amino acids. Atto550-Actin is chemically modified

G-actin with an Atto550 fluorescent group covalently bound to lysine residues.

Atto550-Actin (λem 550nm, λem 576nm) possesses the polymerization properties

of native G-actin.

Conjugation ratio

 

 

 

This product is supplied with a degree of labelling (DOL) between 0.5 and 0.8.

The precise DOL is stated in the DataSheet supplied with the product.

 

Properties

Form

 

Lyophilized, ready-to-use

Quantity

 

1x100 µg, 5x100 µg

Buffer

 

 

 

 

2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3%

disaccharides, when reconstituted with 1.0 ml ultrapure water to obtain a 1mg/ml

solution.

 

Purity

 

 

>99% by scanning densitometry

 

Purification notes

 

 

Purified from rabbit skeletal muscle.

 

Protein conc.

 

 

Determined by the Biuret method.

 

Storage instructions

 

 

 

Atto550-Actin are stored at -70°C upon arrival will be stable in performance for

at least 6 months from the date of purchase. Solubilized Atto-actin is kept on

ice and should be used within 1 week. Avoid refreezing.

 

Shipping conditions

 

 

At ambient temperature. Upon delivery store at -70°C.

 

Remarks

 

 

 

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or

Therapeutical Applications.

 

 

Atto647-Actin for TIRFM (alpha skeletal muscle actin, rabbit)

 

Description

Protein

 

Atto647-Actin

Origin

 

skeletal muscle alpha actin, rabbit

Molecular mass

 

42kDa

Protein description

 

 

 

 

 

Actin from rabbit skeletal muscle is a single chain polypeptide with a molecular

weight of 42kD consisting of 375 amino acids. Atto647-Actin is chemically modified

G-actin with an Atto647 fluorescent group covalently bound to lysine residues.

Atto647-Actin (λem 647nm, λem 669nm) possesses the polymerization properties

of native G-actin.

Conjugation ratio

 

 

 

This product is supplied with a degree of labelling (DOL) between 0.3

The precise DOL is stated in the DataSheet supplied with the product.

 

Properties

Form

 

Lyophilized, ready-to-use

Quantity

 

1x100 µg, 5x100 µg

Buffer

 

 

2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3%

disaccharides, when reconstituted with 1.0 ml ultrapure water to obtain a 1mg/ml

solution.

Purity

 

 

>99% by scanning densitometry

 

Purification notes

 

 

Purified from rabbit skeletal muscle.

 

Protein conc.

 

 

Determined by the Biuret method.

 

Storage instructions

 

 

 

Atto647-Actins are stored at -70°C upon arrival will be stable in performance for

at least 6 months from the date of purchase. The Solubilized product is kept on ice,

protect from light and should be used within 1 week. Avoid refreezing of actin.

 

Shipping conditions

 

 

At ambient temperature. Upon delivery store at -70°C.

 

Remarks

 

 

 

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or

Therapeutical Applications.

 

 

Ordering informations

 

Catalog No.

Product Name

Size

 8162

Atto565-Actin (alpha skeletal muscle actin, rabbit)

1 x 100µg, 5 x 100µg

 8153

Atto488-Actin (alpha skeletal muscle actin, rabbit)

1 x 100µg, 5 x 100µg

 8156

Atto550-Actin (alpha skeletal muscle actin, rabbit)

1 x 100µg, 5 x 100µg

 8158

Atto647-Actin (alpha skeletal muscle actin, rabbit)

1 x 100µg, 5 x 100µg

 

 

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