Hypermol EK
 
작성일 : 14-09-11
[Hypermol EK] Atto-Actin(alpha actin, rabbit skeletal muscle)-II
Atto532-Actin / Atto550-Actin / Atto594-Actin / Atto647-Actin

 

Atto-Actin (alpha actin, rabbit skeletal muscle)_II

 

The Colours of Actin!

The bright way to visualize actin interactions...

 

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Atto-Actins are important tools for investigating actin dynamics.

 

Ready-to-use fluorescent actin conjugates suited for numerous state-of-the-art techniques. Atto-actins are

used for all types of fluorescence microscopes: from epifluorescence to high resolution laser microscopy,

biophysical spectroscipcal methods and biochemical applications. The novel Atto-Actins are characterized

by high photo stabilty, strong absorption and high fluorescence quantum yields - some of several features

demanded for todays imaging techniques.    

 

These fluorescent actin conjugates are prepared by esterification of lysine residues with activated Atto dyes.

Atto-Actins are validated to possess the polymerization properties of native actin.

 

Atto-Actins are worldwide exclusively manufactured by Hypermol.

 

Applications

TIRF Microscopy, FRET, Confocal Microscopy, Epifluorescence Microscopy, Biophysical Spectroscopical

Techniques, Biochemical Actin Interaction Assays...

 

 

Atto532-Actin (alpha actin skeletal muscle, rabbit)

 

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ATTO 532

 

• High fluorescence yield

• High photostability & small Stokes-shift

• Especially suited for Argon-Ion & Nd: YAG laser

 

Description

Protein

 

Atto532-Actin

Origin

 

skeletal muscle alpha actin, rabbit

Molecular mass

 

42kDa

 

Protein description

 

 

 

 

 

 

Actin from rabbit skeletal muscle is a single chain polypeptide with a molecular

weight of 42kD consisting of 375 amino acids. Atto532-Actin is chemically modified

G-actin with an Atto532 fluorescent group covalently bound to lysine residues.

Atto532-Actin (λem 532nm, λem 553nm) possesses the polymerization properties

of native G-actin.

 

Conjugation ratio

 

 

 

This product is supplied with a degree of labelling (DOL) between 0.5 and 0.8.

The precise DOL is stated in the DataSheet supplied with the product.

 

Properties

Form

 

Lyophilized, ready-to-use

Quantity

 

1x100 µg, 5x100 µg

 

Buffer

 

 

 

2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3%

disaccharides, when reconstituted with 1.0 ml ultrapure water to obtain a 1mg/ml

solution.

Purity

 

>99% by scanning densitometry

Purification notes

 

Purified from rabbit skeletal muscle.

Protein conc.

 

Determined by the Biuret method.

Storage instructions

 

 

 

Atto532-Actins are stored at -70°C upon arrival will be stable in performance for

at least 6 months from the date of purchase. Solubilized Atto-actin is kept on ice

and should be used within 1 week. Avoid refreezing.

 

Shipping conditions

 

At ambient temperature. Upon delivery store at -70°C.

 

Remarks

 

 

 

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or

Therapeutical Applications.

 

 

Atto550-Actin (alpha skeletal muscle actin, rabbit)

 

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ATTO 550

 

• High fluorescence yield

• Strong absorption, high photo-stability

• High resolution microscopy, single molecule detection

 

Description

Protein

 

Atto550-Actin

Origin

 

skeletal muscle alpha actin, rabbit

Molecular mass

 

42kDa

 

Protein description

 

 

 

 

 

 

Actin from rabbit skeletal muscle is a single chain polypeptide with a molecular

weight of 42kD consisting of 375 amino acids. Atto550-Actin is chemically modified

G-actin with an Atto565 fluorescent group covalently bound to lysine residues.

Atto550-Actin (λem 550nm, λem 576nm) possesses the polymerization properties

of native G-actin.

 

Conjugation ratio

 

 

 

This product is supplied with a degree of labelling (DOL) between 0.5 and 0.8.

The precise DOL is stated in the DataSheet supplied with the product.

 

Properties

Form

 

Lyophilized, ready-to-use

Quantity

 

1x100 µg, 5x100 µg

 

Buffer

 

 

 

2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3%

disaccharides, when reconstituted with 1.0 ml ultrapure water to obtain a 1mg/ml

solution.

Purity

 

>99% by scanning densitometry

Purification notes

 

Purified from rabbit skeletal muscle, GPC.

Protein conc.

 

Determined by the Biuret method.

Storage instructions

 

 

 

Atto550-Actins are stored at -70°C upon arrival will be stable in performance for

at least 6 months from the date of purchase. Solubilized Atto-Actin is kept on

ice and should be used within 1 week. Avoid refreezing

 

Shipping conditions

 

At ambient temperature. Upon delivery store at -70°C.

 

Remarks

 

 

 

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or

Therapeutical Applications.

 

 

Atto594-Actin (alpha skeletal muscle actin, rabbit)

 

Description

Protein

 

Atto594-Actin

Origin

 

skeletal muscle alpha actin, rabbit

Molecular mass

 

42kDa

 

Protein description

 

 

 

 

 

 

Actin from rabbit skeletal muscle is a single chain polypeptide with a molecular

weight of 42kD consisting of 375 amino acids. Atto594-Actin is chemically modified

G-actin with an Atto594 fluorescent group covalently bound to lysine residues.

Atto594-Actin (λem 601nm, λem 627nm) possesses the polymerization properties

of native G-actin.

 

Conjugation ratio

 

 

 

This product is supplied with a degree of labelling (DOL) between 0.5 and 0.8.

The precise DOL is stated in the DataSheet supplied with the product.

 

Properties

Form

 

Lyophilized, ready-to-use

Quantity

 

1x100 µg, 5x100 µg

 

Buffer

 

 

 

2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3%

disaccharides, when reconstituted with 1.0 ml ultrapure water to obtain a 1mg/ml

solution.

Purity

 

>99% by scanning densitometry

Purification notes

 

Purified from rabbit skeletal muscle.

Protein conc.

 

Determined by the Biuret method.

Storage instructions

 

 

 

Atto594-Actin are stored at -70°C upon arrival will be stable in performance for

at least 6 months from the date of purchase. Solubilized Atto-actin is kept on ice

and should be used within 1 week. Avoid refreezing of actin.

 

Shipping conditions

 

At ambient temperature. Upon delivery store at -70°C.

 

Remarks

 

 

 

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or

Therapeutical Applications.

 

 

Atto647-Actin (alpha skeletal muscle actin, rabbit)

 

Description

Protein

 

Atto647-Actin

Origin

 

skeletal muscle alpha actin, rabbit

Molecular mass

 

42kDa

Protein description

 

 

 

 

 

Actin from rabbit skeletal muscle is a single chain polypeptide with a molecular

weight of 42kD consisting of 375 amino acids. Atto647-Actin is chemically modified

G-actin with an Atto647 fluorescent group covalently bound to lysine residues.

Atto647-Actin (λem 647nm, λem 669nm) possesses the polymerization properties

of native G-actin.

 

Conjugation ratio

 

 

 

This product is supplied with a degree of labelling (DOL) between 0.5 and 0.8.

The precise DOL is stated in the DataSheet supplied with the product.

 

Properties

Form

 

Lyophilized, ready-to-use

Quantity

 

1x100 µg, 5x100 µg

 

Buffer

 

 

 

2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3%

disaccharides, when reconstituted with 1.0 ml ultrapure water to obtain a 1mg/ml

solution.

Purity

 

>99% by scanning densitometry

Purification notes

 

Purified from rabbit skeletal muscle.

Protein conc.

 

Determined by the Biuret method.

Storage instructions

 

 

 

Atto647-Actins are stored at -70°C upon arrival will be stable in performance for

at least 6 months from the date of purchase. Solubilized Atto-actin is kept on ice

and should be used within 1 week. Avoid refreezing of actin.

 

Shipping conditions

 

At ambient temperature. Upon delivery store at -70°C.

 

Remarks

 

 

 

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or

Therapeutical Applications.

 

 

Ordering informations

 

Catalog No.

Product Name

Size

8155

Atto532-Actin(alpha skeletal muscle actin, rabbit)

1 x 100 µg, 5 x 100 µg

8156

Atto550-Actin(alpha skeletal muscle actin, rabbit)

1 x 100 µg, 5 x 100 µg

8157

Atto594-Actin(alpha skeletal muscle actin, rabbit)

1 x 100 µg, 5 x 100 µg

8158

Atto647-Actin(alpha skeletal muscle actin, rabbit)

1 x 100 µg, 5 x 100 µg

 

 

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