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작성일 : 14-07-28
[Array Bridge] Anti-human IgG ELISA kit

Anti-human IgG ELISA kit

The anti-human IgG ELISA kit is designed to quantitatively measure human IgG levels in biological samples.

 

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Principle

 

A series of human IgG standards is provided to generate a standard curve for the assay and all unknown

sample concentrations should be read off this standard curve. IgG standards or diluted unknown samples are

pipetted into the provided 96-well plate which has been pre-coated with anti-human IgG antibodies to capture

IgG molecules from biological samples. Following an incubation to allow capture of the IgG molecules by the

antibodies on the plate, a second anti-human IgG antibody, conjugatedwith biotin, is added and incubated to

allow it to bind to the captured IgG proteins. After a one hour incubation, the plate is washed and a

Streptavidin-HRP (Horse Radish Peroxidase) conjugate is added and incubated for 45 minutes. The

Streptavidin-HRP conjugate will be captured by any biotin labeled antibody bound to the plate. Following a

wash step to remove unbound conjugate, TMB substrate is added and is converted by the captured HRP to a

colored product in proportion to the amount of IgG bound to the plate.After a short incubation to allow color

development, the reaction is stopped and the intensity of the generated color is detected in a

spectrophotometer plate reader capable of measuring 450 nm wavelength. A standard curve will be generated

from the provided IgG standards and used to calculate the concentration of IgG proteins in the unknown

samples, after making suitable correction for the dilution of the sample.

 

Supplied Components

  • Coated Clear 96 Well Plates
  • Human IgG Standards
  • 5x Dilution Buffer
  • 10x PBS-T
  • Reporting antibody
  • Streptavidin-HRP Conjugate
  • TMB Substrate
  • Stop Solution
  • Plate Sealer

 

Product information  (PDF)

 

Ordering information

Catalog No.

Target

Size

AB000303

 Anti-human IgG ELISA kit

1 kit, 5 kits & 20 kits

 

▣ 관련 페이지 ; Array Bridge  (바이오시밀러/Biosimilar 구조 평가 ELISA kits, etc.)