Anti-human Endocan/ESM-1 Antibody(N-Ter) MEP21
Anti-human endocan/ESM-1 antibody MEP21(LIA-0902) detects the N-terminus of human endocan. This antibody is recommended for western-blot applications.
- Clone : MEP21
- Epitope : N-Ter
- Format : 1 tube/100 µg
- Type : Monoclonal(mouse)
- Application : WB
- Isotype : IgG2a, K
* The monoclonal MEP21 antibody(LIA-0902) can be ordered in custom sizes (bulk).
Endocan also known as endothelial cell-specific molecule (ESM-1) was originally discovered by Lassalle and colla-borators in endothelial cells (Lassalle et al. 1996). Structurally, endocan is a dermatan sulfate proteoglycan of 50 kDa that is freely circulating in blood (Bechard et al. 2001a; Delehedde et al. 2002). Endocan binds CD11a/CD18 integrin (also called LFA-1 for Leukocyte Function-associated Antigen-1) on human leukocytes inhibiting consequently its binding to ICAM-1 (Bechard et al. 2001b ; Sarrazin et al. 2010b). The expression of endocan / ESM-1 is upregulated by pro-inflammatory molecules such as tumor necrosis factor alpha (TNFa), lipopolysaccharides (LPS) and also pro-angiogenic molecules such as vascular endothelial growth factor (VEGF), fibroblast growth factor 2 (FGF-2) (Grigoriu et al. 2006; Sarrazin et al. 2006; Maurage et al. 2009 ; Leroy et al. 2010 ; Sarrazin et al. 2010). Endocan / ESM-1 binds via its dermatan sulfate chain to hepatocyte growth factor/scatter factor (HGF/SF) (Bechard et al. 2001a ; Sarrazin et al. 2010b). Recently, endocan / ESM-1 has been described as a specific biomarker of tip cells in neoangiogenesis (Strasser et al. 2010 ; Del Toro et al. 2010). Elevated blood levels of endocan / ESM-1 has been reported in patients with lung and kidney cancers as well as in patients with severe sepsis (Bechard et al. 2001b; Scherpereel et al. 2003; Grigoriu et al. 2006; Scherpereel et al. 2006 ; Leroy et al. 2010).
Main Features :
- Monoclonal Antibody (mouse)
- Detect human endocan
- Detect by immunoblotting a 50 kDa band corresponding of proteoglycan
- Detect the N-terminus of human endocan
- Recommended reference for immunoblotting (WB)
* This product is "for research use only and are not intended for diagnostic or therapeutic use".
Grigoriu et al. (2006) Clin. Cancer Res. 12:4575-4582.
Sarrazin et al. (2006) BBA Reviews 1765:25-37.
Sarrazin et al. (2010) J. Cancer. Sci. Ther. 2:47-52.