Lunginnov
 
ۼ : 12-05-07
[Lunginnov] Anti-human Endocan/ESM-1 Antibody(N-Ter) MEP08
Anti-human Endocan/ESM-1 Antibody(N-Ter) MEP08
 
 
Anti-human endocan/ESM-1 antibody clone MEP08 (LIA-0901) detects the N-terminus of human, chicken and monkey
endocan / ESM-1. This monoclonal antibody is recommended for IHC applications.
 
 
Informations
  • Clone : MEP08
  • Epitope : N-Ter
  • Format : 1 tube/100 µg
  • Type : Monoclonal(mouse)
  • Application : IHC
  • Isotype : IgG2a, K
* The monoclonal MEP08 antibody (LIA-0901) can be ordered in custom sizes (bulk).
 
 
Background

Endocan also known as endothelial cell-specific molecule (ESM-1) was originally discovered by Lassalle and colla-
borators in endothelial cells (Lassalle et al. 1996). Structurally, endocan is a dermatan sulfate proteoglycan of 50 kDa that
is freely circulating in blood (Bechard et al. 2001a; Sarrazin et al. 2010b). Endocan binds CD11a/CD18 integrin (also called
LFA-1 for Leukocyte Function-associated Antigen-1) on human leukocytes inhibiting consequently its binding to ICAM-1
(Bechard et al. 2001b). The expression of endocan / ESM-1 is upregulated by pro-inflammatory molecules such as tumor
necrosis factor alpha (TNFa), lipopolysaccharides (LPS) and also pro-angiogenic molecules such as vascular endothelial
growth factor (VEGF), fibroblast growth factor 2 (FGF-2) (Grigoriu et al. 2006; Sarrazin et al. 2006; Maurage et al. 2009
; Leroy et al. 2010 ; Sarrazin et al. 2010a). Endocan / ESM-1 binds via its dermatan sulfate chain to hepatocyte growth
factor/scatter factor (HGF/SF) (Bechard et al. 2001a ; Sarrazin et al. 2010a). Recently endocan / ESM-1 was identified as a
biomarker of tip cells (Strasser et al. 2010 ; Del Toro et al. 2010). Elevated blood levels of endocan / ESM-1 has been
reported in patients with lung and kidney cancers as well as in patients with severe sepsis (Bechard et al. 2001b;
Scherpereel et al. 2003; Grigoriu et al. 2006; Scherpereel et al. 2006 ; Leroy et al. 2010). Endocan is a biomarker of
activated endothelium (Sarrazin et al. 2010b).
 
 
Instruction sheet download :  Click here
  
Download Datasheet :  Click here
 
 
Main Features
  • Monoclonal Antibody (mouse)
  • Detect human and monkey endocan
  • Recommanded for immunohistochemistry (paraffin and frozen sections)
  • Specific of the N-terminus of human endocan / ESM-1
  • Easy to use / do not required any enzymatic pre-treatment
  • Can be use with automatized procedures (see protocols sections)
        * This product is "for research use only and are not intended for diagnostic or therapeutic use".
 
 
References
 
Bechard et al. (2000) J. Vasc. Res. 37:417-425 (Pubmed)
Grigoriu et al. (2006) Clin. Cancer Res. 12:4575-4582 (Pubmed)
Maurage et al. (2009) Exp. Neurol. 68:836-844 (Pubmed)
Leroy et al. (2010) Histopathology 56:180-187 (Pubmed)
Sarrazin et al. (2010) J. Canc. Sci. Ther. 2:47-52 (Pubmed)