Biovica International AB
작성일 : 14-04-05
[Biovica International AB] 혈청 중 Thymidine Kinase 활성을 고감도 측정 kit
Quantitative determination of thymidine kinase (TK) activity in serum

혈청 중의 TK(Thymidine Kinase) 활성을 고감도로 측정하는 kit ,  DiviTum™ Kit

             클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

혈청 중의 TK(Thymidine Kinase) 활성을 방사성 동위원소(radioactive technique)를 사용하지 않고, 비색법에 의해 매우 고감도로 측정 할 수 있는 제품입니다. 혈청 중 TK 활성 상승은 종양질환 및 악성질환에 관련된 것으로 보고되고 있어, 세포분열 활동의 지표인 TK 활성을 측정함으로써 종양의 사전 예방 및 조기 치료와 치료의 효율성을 관찰하는 도구로써 사용될 수 있습니다.



TK is an enzyme converting deoxythymidine to deoxythymidine monophosphate. The main isoenzyme in the

circulation is expressed in the G1-S cell division stage to supply the thymidine triphosphate necessary for cell division.

Presence of active TK in serum and its correlation to tumour disease was shown 1982. Tests with lower sensitivity

from other manufacturers are used routinely in clinical laboratories. The following reasons for elevated TK levels in

serum have been suggested: in addition to malignant disease also vitamin B12 deficiency, and certain virus infections.

With the high sensitivity obtained with DiviTum®, transient elevations during wound healing after e.g. surgery have

been reported. TK activity has also been found in body fluids other than serum, such as cerebrospinal fluid, pleural

fluid and ascitic fluid.

Assay Principle of DiviTum®

As substrate for TK the DiviTum® assay uses bromo-deoxyuridine which is phosphorylated to its monophosphate. To

immobilize and remove the monophosphate from the solution it is further phosphorylated to the tri-phosphate by

other kinases present in the reaction solution. The tri-phosphate is immobilized by DNA synthesis, in which a polyA

strand covalently bound to the microtiter plate well acts as template, reverse transcriptase as the catalyst and odT as

the primer. After the TK activity incubation is completed, the plate is washed and incubated with an anti-

bromodeoxyuridine-antibody conjugated to alkaline phosphatase followed by a second wash. The amount of alkaline

phosphatase is evaluated using a chromogenic substrate, p-nitrophenyl phosphate (pNPP). The assay measures the

activity of the enzyme. The TK activity is given as Du/L. 1000 Du/L corresponds to the activity in a solution

containing 1000 ng/L of a reference sample of recombinant TK.


DiviTum™ – Important Features

• Measures the growth rate of a tumor with unique sensitivity and precision – using a simple blood sample

• A predictive marker for therapy response, disease progression and overall survival in solid tumors

• Demonstrates precise prognostic capabilities in several forms of solid tumors

• Can be an attractive companion diagnostic for pharma companies in research projects when evaluating

   pipeline drugs


Important areas of use

One of the most important applications for the TK test has historically been in non-Hodgkin’s lymphoma and in

chronic lymphocytic leukaemia. TK levels can give an early warning of recurrence of the disease, allowing timely


In myelodysplastic syndrome, the TK value is fundamental for the choice of treatment, as it gives a reliable indication

of the risk for transformation to acute myeloid leukaemia.

In patients with breast cancer, the DiviTum™ value can predict response to therapy and outcome at certain cut-off

levels, as demonstrated in a recent trial by Karolinska Institutet. This information can affect the choice of treatment

and facilitate tailored therapy for the patient.


Assay characteristics

The assay defines the TK activity on the basis of the amount of DNA produced and quantified using an ELISA

technique. The TK activity is not strictly proportional to the TK concentration present and thus the assay should

always be performed using the amount of serum and incubation times given in this Instruction for Use. The DiviTum®

assay has an analytical sensitivity of 10 Du/L (LLD) / 50 Du/L (LLQ). The working range extends up to 5000 Du/L.

The precision (CV) at 100Du/L is better than 20%.


클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

Fig 1. Typical standard curve after 3 hours incubation time


클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

Figure 2. shows the distribution of serum TK activity of 240 apparently healthy blood donors in 12 age groups

(10 men and 10 women in each group), measured with the DiviTum® kit.


클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

Figure 3. Correlation between values obtained with DiviTum® and Prolifigen® for tumour bank samples analysed.


Reference Range

The data in figure 2 show that the 90th percentile corresponds to approximately 80 Du/L and the 80th percentile to

approximately 50 Du/L. This was a closed study and a second sample to exclude transient TK increase could not be

obtained. Half of the blood donors were over 50 years old. Therefore, it is probable that a certain proportion of them

have tumour disease not yet diagnosed but still influencing their TK values and also that there are some cases of viral

infections among the blood donors. This makes it reasonable to use the 80th percentile, corresponding to 50 Du/L,

as the upper limit of the reference range.


Kit Components

  • Adhesive tape cover
  • Microtiter plate lid
  • Pipetting tray
  • Sample dilution plate with 96 wells
  • Immunosorbent microtiter plate with 96 wells
  • Sample diluent - ready to use
  • Reagent A / B
  • Control low / high
  • Solution - ready to use
  • Washing buffer - 80 x concentrate
  • Enzyme conjugate diluent
  • Enzyme conjugate
  • Substrate buffer
  • Enzyme conjugate substrate

* Each kit contains materials for analysis of 38 samples including controls in duplicate (2×10 µL is required of

   each sample).


Brochure (PDF)

Instruction sheet (PDF)

Clinical Study  (Published studies)



Background and use in gene therapy (PDF)

The quantitative assay for TK and its clinical use (PDF)

Use of TK assay in serum, plasma and other body fluids (PDF)


Ordering informations

Catalog No.

Product Name



DiviTum® kit

38 samples in duplicate / kit


DiviTum® kit

10 kits