AparnaBio
 
작성일 : 14-02-20
[AparnaBio] InVitroPlex®, Suspension Culture Transfection
InVitroPlex®, Suspension Culture Transfection
 
 
InVitroPlex™ Express
Low Cost Suspension Culture Transfection
 
Generate Proteins, mAb, Viral Vectors, VLP, ...
 
Advanced Nanoparticle Transfection
  • High efficiency: High plasmid expression with HEK293F cells
  • Low toxicity: Healthy cells express higher levels, and for longer periods
  • Simple application: Media changes are not necessary
  • Very affordable: Low cost provides substantial savings over popular reagents
 
High protein expression levels
 
AparnaBio’s InVitroPlex™ Express produced high levels of intracellular luciferase, comparable to levels by an expensive
popular commercial reagent, but lower cost.  They are available in formulations optimized for suspension cultures of
HEK293 cells, for CHO cells, and a formulation optimized for adherent cultures.
 
 
High Level of Luciferase Expression in Suspension Culture
클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요
Fig. 1.  Luciferase activity 24 h after transfection of 293F cell suspension culture in Pro293s-CDM media.  Note that
InVitroPlex™ Express gave high Luc expression comparable to that by a popular reagent.
 
 
Nanoparticle with low toxicity – simple to use with great results
 
InVitroPlex™ Express is a low cost, yet fully synthetic nanoparticle transfection reagent, optimized for 293F suspension
culture using serum free or serum containing media, with low cyto-toxicity.  Also, it is simple to use as there is no
requirement for media changes, and thus an excellent choice for in vitro transfection production of proteins, mAb, viral
vectors, VLPs, etc. for small and large scale research.
 
 
InVitroPlex™ ExpressPlus
Advanced Suspension Culture Transfection
 
Generate Proteins, mAb, Viral Vectors, VLP, ...
 
From Research to Bioproduction
 
High Efficiency & Enabling Translation Research
  • High Efficiency: High plasmid expression in HEK293F suspension cells
  • Biodegradable: No chemical residuals, improves product quality
  • Low Toxicity: Healthy cells express higher levels, and for longer periods
  • Versatile: High expression across common serum free media, or with serum
  • Scalable: Small to large volume cell culture, support for cGMP production
 
High protein expression levels
 
AparnaBio’s InVitroPlex™ ExpressPlus produced high levels of intracellular luciferase as compared to levels by a popular
commercial reagent:
 
 
High Level Luciferase Expression by 293F Suspension Culture
 
클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요
Fig. 2.  Luciferase activity 24 h after transfection of 293F cell suspension culture in Pro293s-CDM media.  Note that
InVitroPlex™ ExpressPlus gave higher Luc expression when compared to a popular reagent.
 
Unique biodegradable nanoparticle reagent with low toxicity
 
InVitroPlex™ ExpressPlus is a biodegradable nanoparticle transfection reagent optimized for 293F suspension culture
across commonly used serum free or serum containing media, with low cyto-toxicity, when used according to the protocol.
 
Versatile and scalable for bioproduction  
 
InVitroPlex™ ExpressPlus is a fully synthetic polyamide reagent without any biological material, and scalable to cGMP,
making it an excellent choice for production from early research to clinical testing of proteins, mAb, viral vectors, VLPs,
etc.
 
 
InVitroPlex™ -Express Parasite
 
Transfection Reagents for Tropical Parasites and Snail Hosts
 
Express Proteins, Inhibit Genes with siRNA, or Deliver miRNA
  • Broad efficiency: plasmid expression in parasites or snail hosts
  • Low toxicity: achieve specific effects of gene modulation
  • Simple application: incubate living tissue in tissue culture
  • Very affordable: low cost and compatibility with multiwell studies
 
AparnaBio’s InVitroPlex™ -Express Parasite is an advanced polymer reagent with an improved buffer formulation that has
been used by leading laboratories in the tropical parasite research field.
 
 
Ordering inforamtions
 
Catalog No.
Product Name
Size
IVP-EX-001
InVitroPlex Express
0.5 ml
IVP-EX-002
2.0 ml
IVP-EX-003
10.0 ml
IVP-EXPA-001
InVitroPlex Express Parasite
0.5 ml
IVP-EXPA-002
2.0 ml
IVP-EXPA-003
10.0 ml
* InVitroPlexTM Express reagents are used at a ratio of 2 ul/ug nucleic acid.  The separate InVitroPlexTM Enhancing Buffer
   is the same amount provided in the 2 ml InVitroPlex kits.  For larger reagent amounts or animal studies, please contact us.
 
 
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