Biotech Support Group
 
작성일 : 14-01-08
[Biotech Support Group] Kinase & ATP binding proteins enrichment reagents
 
KinaSorb™ Kinase (& ATP binding proteins) enrichment reagents
 
 
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  • Ratio of Total Kinase Activity* relative to total protein content increases 300% or more
  • ATP binding protein recoverable, ~200 ug
  • Up to 4X concentration of kinase volume, relative to starting volume
  • 60 minute, scaleable protocol compatible with functional assays, electrophoresis and Mass Spec
  • Phosphatase (neutral & alkaline) activity not detectable
  • Improves protein normalization when comparing heterogeneous tissues
 
KinaSorb™ is a new reagent kit used for the enrichment and isolation of kinases. In a patent pending process, ATP – the
common substrate for kinases, is reversibly immobilized to metallic oxide particles, producing a single-use highly efficient
enrichment method for kinase & other ATP binding proteins. The standard prep protocol starts with 100 µl of clarified cellular extracts, or approximately 2 mg total cellular extract protein, but the process can be scaled up or down to accommodate
different sample volumes and protein concentrations. The methods are reasonably specific to ATP; little Phosphodiesterase
activity (an enzyme which binds to a structurally similar adenosine containing substrate - CAMP), was measured in the eluant after KinaSorb™ treatment. The kit includes all necessary reagents for immediate use.
 
 
Ordering information
 
Catalog No.
Product Name
Size
 KE785 (PDF)
KinaSorb™ Kinase
10 (0.5 ml) & 50 (2.5 ml) preps
 
 
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