Bayou Biolabs
 
작성일 : 13-12-23
[Bayou Biolabs] Ready to Ligate pUC18-SmaI-AP and M13mp18-SmaI-AP
 
Ready to Ligate pUC18-SmaI-AP and M13mp18-SmaI-AP
 
 
Highly Purified: Prepared from pUC18 or M13mp18-SmaI by restriction digestion, alkaline phosphatase dephosphorylation, purification by agarose gel electrophoresis, and further purification to remove all residual agarose. Our competitors purify the linear plasmid by gel filtration chromatography, which does not remove residual supercoiled and nicked plasmid, resulting in high backgrounds. Our product is purified by agarose gel electrophoresis, which does remove all residual supercoiled and
nicked plasmid, resulting in low backgrounds.
 
Ready immediately for ligation: The dephosphorylated vectors can be ligated to any phosphorylated DNA for cloning.
 
Rigorously Quality Controlled to ensure low background transformation and intact restriction site ends.
     
Excellent starting material for making double restriction digested vector for directional cloning. An easy two hour protocol is included for making double restriction digested plasmid from Ready to Ligate plasmid.
 
Supplied in 100 ul TE at 100 ng/ul, a convenient concentration for routine use.
 
 
Ordering informations
 
Catalog No.
Product Name
Size
P-204
pUC18-SmaI-AP
10 ug
P-224
M13mp18-SmaI-AP
10 ug
 
 
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