Biotech Support Group
 
작성일 : 13-12-03
[Biotech Support Group] Proteomics Separations and Enrichment kits
NuGel™ PROspector™ / NuGel™ PROfessor™
 
Proteomics Separations and Enrichment kits
 
NuGel™ PROspector™
Enrichment of fuctional biomarkers
 
 
클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요
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  • Efficiently produce up to 12 differentiated subproteomes with uncompromised functional and structural integrity
  • Generate characteristic molecular profiles for comparison and discovery
  • Enrich functional biomarkers for sequence and structural annotation
  • Kit includes 6 mixed mode surface chemistries per prep
 
Functional proteomics relies in part, on the functional or structural features of intact, non-denatured proteins. While the
terminology can often overlap, chemical and affinity-based proteomic profiles can be considered a subset of functional
proteomics. Thus, functional proteomic annotation may complement conventional sequence annotation while supporting the study of mechanism of action and drug promiscuity. Furthermore, the subtleties of protein attributes, when the
same or similar underlying sequence can have multiple conformations and functions, and when different sequences
sometime perform the same or similar functions, are now open to investigation. A new functional proteomics separations toolset based on the NuGel™ passivated porous silica platform, can be used in unrestricted workflow strategies, so as to sift
through these biological complexities.
  • Separations readily compatible with virtually all proteomic interrogations
  • Microtube kit, simple bind/wash/elute protocols
  • No specialized instruments, or HPLC required
  • Disposable, no column regeneration
  • Tryptic digestion or enzyme assay can be ‘on-bead’
  • Universal, species and tissue type agnostic
 
 
NuGel™ PROfessor™
Compound Centric Chemical Proteomics (CCDP) & Protein Compression
 
 
클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요     클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요     클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요
      큰 그림 보기  (Enlarge)                            큰 그림 보기  (Enlarge)                           큰 그림 보기  (Enlarge)
  
 
Enablement for compound-centric displacement proteomics (CCDP)
 
- Non-covalently binds proteins, a subset of which can be displaced upon introduction of soluble small compounds
- Coupled to LC-MS, spectral counts of affinity-eluted pools can serve as quantitative metrics to characterize and identify
   interacting proteins
- Gain efficiencies over prior covalent-based substitution methods: more complete survey, no orientation bias, robust
   background controls
- Applications in drug target deconvolution, on-target/off-target specificity, and personalized medicine
 
Can compress protein concentrations, enrich low abundance proteins
  • Function, structure and sequence can be characterized and correlated
  • Low abundance proteome enriched 5-10X
  • Tissue and species agnostic
  • Suitable for small volume mg scale preps
  • Composite PROspector™ surfaces can be deconstructed and optimized for biomarker enrichment
 
Functional proteomics relies in part, on the functional or structural features of intact, non-denatured proteins. While the
terminology can often overlap, chemical and affinity-based proteomic profiles can be considered a subset of functional
proteomics. Both NuGel ™ PROspector™ and PROfessor™ support functional and chemical proteomics and can:
  • Optimize drug compounds
  • Survey promiscuity
  • Elucidate mechanism of action
  • Identify phenotypic biomarkers
 
 
Ordering informations
 
Catalog No.
Product Name
Size
NuGel™ PROspector™
5 & 25 preps
NuGel™ PROfessor™
10  &  50 preps
 
 
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