Protein Elution Plate(PEP) Universal Protein Purification kit
Proteins play essential roles in numerous biological processes. It is always a challenge to purify a functional protein from a
protein mixture because of their charge and size heterogeneity. Another challenge in protein purification is the limitation of
the amount of material that can be used for the protein purification; sometimes only micrograms of proteins are available,
making most of the current protein purification procedures less effective.
In the PEP technology, protein mixture is first separated by a modified one-dimensional or two-dimensional gel
electrophoresis, this modified procedure provides good resolution while still maintaining protein function. This is followed by
an efficient protein transfer step to a specially designed 384-well Protein Elution Plate (PEP). After further transfer of the
samples from the PEP plate to a master 384-well microplate, functional assays can be performed using part of the sample
from each well to generate an enzyme activity profile. The purity of the protein in each well can be tested by a standard SDS-PAGE (Polyacrylamide Gel Electrophoresis) after loading a portion of the sample from the master 384-well plate. If desired,
Mass Spectrometry can be used to identify the protein from the well with pure protein or from the protein band on the SDS-
PAGE gel. It is also possible to analyze multiple enzyme families in parallel to obtain functional profiles for each enzyme. The PEP technology
can be applied to the systematic analysis of any proteins for which functional assays exist.
PEP Universal Protein Purification kit (Small format)
For fast purification of functional proteins from a fraction or proteome and systematic analysis of enzyme families
Purification of functional protein from modified 1-D SDS-PAGE
PEP Universal Protein Purification kit (Large format)
Complete kit for the fast purification of any functional protein from a protein mixture or a proteome with hundreds to
thousands of proteins. It can also be used to build the three-dimentional enzyme landscape for any protein family for which a functional assay is available such as protein kinases, protein phosphatases, proteinases, oxido-reductases, etc.
Enzyme profile 3-D Landscape and purification of functional proteins from the proteome
▣ 관련 페이지 ; Array Bridge (바이오시밀러/Biosimilar 구조 평가 ELISA kits, etc.)