Array Bridge
 
작성일 : 13-08-23
[Array Bridge] Residual Protein A ELISA Kit
 
Residual Protein A ELISA Kit
Sensitive quantification of residual Protein A (rProtein A) in monoclonal antibody therapeutics  
 
 
 
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Principle
 
The Protein A ELISA kit is designed to quantitatively measure residual Protein A contamination in bulk products after
Protein A purification processes. Please read the complete kit insert before performing this assay. The assay
utilizes an assay plate which has been pre-coated with rabbit anti-Protein A antibodies. The test samples along
with the control samples are pre treated by heat denaturation to remove IgG and IgG-like proteins. It is important to
remove IgG proteins prior to performing this assay as Protein A – IgG complexes (which are very stable) can
prevent recognition of Protein A by the capture antibody on the plate. After the immobilized antibody captures
residual Protein A present in the samples, the captured Protein A or Protein A like proteins is detected by biotin
labeled detection antibody, followed by addition of Streptavidin peroxidase conjugate. This conjugate binds strongly
to the biotin molecule on the detection antibody. The level of bound peroxidase conjugate present in each assay
well is determined by addition of a Peroxidase substrate (TMB), which is acted upon by the peroxidase enzyme to
generate a blue coloration. After a short incubation (10-15 minutes), the reaction is stopped by the addition of acid
solution that generates a yellow coloration. The absorbance of each well in the assay plate at 450 nm is then
recorded using a suitable plate reader.
A series of Protein A standards are provided to generate a standard curve for the assay and all unknown sample
concentrations should be read off this standard curve. It is not necessary to boil these standards as they are already free of contamination IgG molecules.
 
 
Supplied Components
 
  • Coated Clear 96 Well Plates
  • Protein A Standards
  • 5x Dilution Buffer
  • 10 x PBS-T
  • Reporting antibody
  • Streptavidin-HRP Conjugate
  • TMB Substrate
  • Stop Solution
  • Plate Sealer
 
 
 
 
Ordering information
 
Catalog No.
Product Name
Size
AB000109
Residual Protein A ELISA Kit
96 tests
 
 
▣ 관련 페이지 ; Array Bridge  (Biosimilar 구조 평가 ELISA kits, etc.)