MTTI
 
작성일 : 13-08-23
[MTTI] Fluorescent Dye Kits for Cell Membrane Labeling
CellVue®
 
CellVue®, Fluorescent Dye Kits for Cell Membrane Labeling
 
 
CellVue® dyes are fluorescent probes for irreversible labeling of plasma membranes of live cells.
 
 
Features
  • Versatility – use with any cell type or bioparticle with a membrane
  • Provides stable labeling with minimal transfer from cell to cell
  • Provides rapid, uniform membrane labeling
  • Combine with fluorescent antibodies or markers of cell function
  • Suitable for cell tracking and proliferation studies
  • Several colors (UV to NIR) for multi-parameter studies (use with existing fluorochromes for more colors)
  • Far-Red and NIR versions can provide greater signal to noise due to reduced background auto-fluorescence
  • Compatible with flow cytometers, confocal and in vivo imaging equipment
  • Convenient, easy-to-use kit format
 
The CellVue® cell linker kits use proprietary membrane labeling technology to stably incorporate a fluorescent dye with
long aliphatic tails into the lipid regions of the cell membrane, see Figure 1. The labeling vehicle provided with the kit
(Diluent C) is an iso-osmotic aqueous solution which contains no physiologic salts or buffers, detergents, or organic
solvents and is designed to maintain cell viability while maximizing dye solubility and staining efficiency. The pattern of
staining is dependent upon the cell type being labeled and the membranes of the cells.
 
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                                                           Figure 1.
 
CellVue® Maroon
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The CellVue Maroon® Fluorescent Cell Linker Kit contains a 1mM dye stock solution and cell
labeling diluent. Dye fluorescence properties: Ex max =647nm and Em max =667nm. Provides
stable labeling of the lipid regions of cell membranes.
 
CellVue® Claret
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The CellVue Claret Fluorescent Cell Linker Kit contains a 1 mM dye stock solution and cell
labeling diluent. Dye fluorescence properties: Ex max = 655 nm and Em max = 675 nm.
Provides stable labeling of the lipid regions of cell membranes.
 
CellVue® Plum
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The CellVue Plum Fluorescent Cell Linker Kit contains a 1mM dye stock solution and cell
labeling diluent. Dye fluorescence properties: Ex max =652nm and Em max =671nm.
Provides stable labeling of the lipid regions of cell membranes.
 
CellVue® Burgundy
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The CellVue Burgundy Fluorescent Cell Linker Kit contains a 1mM dye stock solution and cell
labeling diluent. Dye fluorescence properties: Ex max =683nm and Em max =707nm.
Provides stable labeling of the lipid regions of cell membranes.
 
CellVue® Lavendar
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The CellVue Lavender Fluorescent Cell Linker Kit contains a 1mM dye stock solution and cell
labeling diluent. Dye fluorescence properties: Ex max =425nm and Em max =461nm.
Provides stable labeling of the lipid regions of cell membranes.
 
CellVue® NIR815
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The CellVue NIR815 Fluorescent Cell Linker Kit contains a 1mM dye stock solution and cell
labeling diluent. Dye fluorescence properties: Ex max =786nm and Em max =814nm.
Provides stable labeling of the lipid regions of cell membranes.
 
CellVue® NIR780
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The CellVue NIR780 Fluorescent Cell Linker Kit contains a 1mM dye stock solution and cell
labeling diluent. Dye fluorescence properties: Abs max =745nm and Em max =776nm.
Provides stable labeling of the lipid regions of cell membranes.
 
Diluent C
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Six vials each containing 10 mL of Diluent C for membrane labeling with CellVue dyes.
 
CellVue® Jade
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The CellVue Jade Fluorescent Cell Linker Kit contains a 1 mM dye stock solution and cell
labeling diluent. Dye fluorescence properties: Abs max = 478 nm and Em max = 508 nm.
Provides stable labeling of the lipid regions of cell membranes.
 
CellVue® Red
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The CellVue Red Fluorescent Cell Linker Kit contains a 1 mM dye stock solution and cell labeling
diluent. Dye fluorescence properties: Ex max = 567 nm and Em max = 588 nm.
Provides stable labeling of the lipid regions of cell membranes.
 
CellVue® Lilac
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The CellVue Lavender Fluorescent Cell Linker Kit contains a 1 mM dye stock solution and cell
labeling diluent. Dye fluorescence properties: Ex max = 423 nm and Em max = 471 nm.
Provides stable labeling of the lipid regions of cell membranes.
Click here for video on how to use CellVue® CellVue Lilac is an improved version of CellVue
Lavender. It has improved dye solubility resulting in improved membrane staining.
 
 
 
Methods for Cell Labeling with CellVue® Dyes
Mixing Steps and Critical Preparation Points
 
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Ordering informations
 
Catalog No.
Product Name
Size
CellVue® Maroon
Mini, Midi & Maxi
CellVue® Claret
Mini, Midi
CellVue® Plum
Mini, Midi & Maxi
CellVue® Burgundy
Mini, Midi & Maxi
CellVue® Lavendar
Mini, Midi & Maxi
CellVue® NIR815
Mini, Midi & Maxi
CellVue® NIR780
Mini, Midi & Maxi
Diluent C
-
CellVue® Jade
Mini, Midi & Maxi
CellVue® Red
Mini, Midi & Maxi
CellVue® Lilac
Mini, Midi & Maxi
* Mini Kit (small): 0.1 ml of 1mM ethanolic dye stock and 1 x 10mL of diluent.
  Midi Kit (medium): 0.2 ml of 1mM ethanolic dye stock and 6 x 10 ml of diluent.
  Maxi Kit (large): 0.5 ml of 1mM ethanolic dye stock and 6 x 10 ml of diluent.
 
 
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