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작성일 : 13-08-19
[Bayou Biolabs] A Breakthrough in Plasmid Purification
Exonuclease-It™
 
Exonuclease-It™, A Breakthrough in Plasmid Purification
 
 
Description: The Exonuclease-It™ plasmid purification system is designed to selectively digest linear chromosomal DNA
contamination in a plasmid preparation, without significantly degrading any supercoiled plasmid (or any covalently closed
circular plasmid). Exonuclease-It™ will also degrade open circular plasmid and circular single stranded DNA. The
Exonuclease-It™ enzyme reagent contains a proprietary mixture of exonucleases to accomplish this task. In a typical
6 hour incubation, Exonuclease-It™ will reduce the amount of chromosomal DNA contamination in a plasmid preparation
by 98 percent. The by-products of the exonuclease digestion are deoxynucleoside monophosphates. The Exonuclease-
It™ plasmid purification system is ideally used as an added step in any plasmid purification method, in order to thoroughly
remove chromosomal DNA. This kit contains sufficient reagents for purifying 150 mg of plasmid. Exonuclease-It™ is not
for use in purifying plasmid for human therapy.
 
Applications: The primary application is for removal of chromosomal DNA contamination from plasmid preparations.
However, Exonuclease-It™ may be used in any application to selectively digest linear DNA without significantly degrading
covalently closed circular double stranded DNA. For example, a mixture of linear DNA and covalently closed circular DNA
may be generated by a ligation reaction. Exonuclease-It™ can selectively digest the linear DNA vector prior to
transforming cells, in order to reduce background transformants. Exonuclease-It™ may be used after Supercoil-It™.
The Supercoil-It™ incubation converts open circular plasmid to supercoiled form, and the subsequent Exonuclease-It™
incubation removes chromosomal DNA contamination.
 
Supplied: Exonuclease-It™ is supplied with sufficient 40X buffer.
 
Low Price:  Purifies 150 mg of plasmid for only $200.
 
Shipping: dry ice.
 
 
 
 
Ordering informations
 
Catalog No.
Product Name
Size
X-101
Exonuclease-It™
purifies 150 mg plasmid
X-102
Exonuclease-It™,
purifies 750 mg plasmid
X-103
Exonuclease-It™,
purifies 3,000 mg plasmid
 
Exonuclease-It™ and Supercoil-It™ are trademarks of Edward David Hyman
 
 
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