Smartox Biotechnology
 
작성일 : 13-07-15
[Smartox Biotechnology] 각종 Acetylcholine Receptor Blocker Peptide Toxin
Waglerin 1 / α-Conotoxin MI / α-Conotoxin GI / α-Conotoxin IMI
 
화학합성의 각종 Acetylcholine Receptor Blocker Peptide Toxin
 
Acetylcholine receptor 연구에 유용한 유독 생물 유래의 합성 peptide toxin 입니다. 화학합성 peptide이기 때문에
endotoxin 오염의 걱정이 없습니다.
 
 
Waglerin 1
 
Waglerin 1 (Wtx-1) is a peptide originally isolated from the venom of the Wagler's pit viper (Trimeresurus wagleri). This
22 amino-acid peptide is a competitive antagonist of muscle nicotinic acetylcholine receptors. Waglerin 1 binds selectively to epsilon subunit of nAChR. Some studies have demonstrated that Waglerin 1 has an effect on ionotropic GABA
receptors. It may potentiate or depress I(GABA) depending on the neurons. Some derivatives of Waglerin 1 are
currently used in cosmetics to reduce wrinkles.
 
Details
AA sequence:
 
Gly-Gly-Lys-Pro-Asp-Leu-Arg-Pro-Cys9-His-Pro-Pro-Cys13-His-Tyr-Ile-Pro-Arg-Pro-Lys-Pro-Arg-OH
 
 
(Disulfide bonds between Cys9-Cys13)
Length (aa):
 
22
Formula:
C112H175N37O26S2
Molecular Weight:
 
2520 Da
Appearance:
 
White lyophilized solid
Solubility:
 
Water and saline buffer
CAS number:
NA
Source:
 
Synthetic
 
 
 
 
References
1. Teichert RW. et al. Peptide-toxin tools for probing the expression and function of fetal and adult subtypes of the
    nicotinic acetylcholine receptor. Ann N Y Acad Sci. PubMed link
2. Sellin LC. et al. Conformational analysis of a toxic peptide from Trimeresurus wagleri which blocks the nicotinic
    acetylcholine receptor. Biophys J. 1996. PubMed link
3. Molles BE. et al. Identification of residues at the alpha and epsilon subunit interfaces mediating species
    selectivity of Waglerin-1 for nicotinic acetylcholine receptors. J Biol Chem. 2002. PubMed link
4. McArdle JJ. et al. Waglerin-1 selectively blocks the epsilon form of the muscle nicotinic acetylcholine receptor.
   J Pharmacol Exp Ther. PubMed link
5. Ye JH. et al. Waglerin-1 inhibits GABA(A) current of neurons in the nucleus accumbens of neonatal rats.
    Brain Res. 1999. PubMed link
6. Ye JH. et al. Waglerin-1 modulates gamma-aminobutyric acid activated current of murine hypothalamic neurons.
    J Pharmacol Exp Ther. PubMed link
 
 
α-Conotoxin MI / Alpha conotoxin MI
 
α-conotoxin MI has been isolated from the venom of the cone snail Conus magus. This conotoxin acts on postsynaptic
membranes.
It binds onto and blocks nicotinic acetylcholine receptors (nAChR).  α-conotoxin MI blocks mammalian nAChR composed
of α1/δ subunits with high potency and α1/γ with low potency.
 
Details
AA sequence:
 
H-Gly-Arg-Cys3-Cys4-His-Pro-Ala-Cys8-Gly-Lys-Asn-Tyr-Ser-Cys14-NH2
 
 
(Disulfide bonds between Cys3-Cys8 and Cys4-Cys14)
Length (aa):
 
14
Formula:
C58H88N22O17S4
Molecular Weight:
 
1495. 08 Da
Appearance:
 
White lyophilized solid
Solubility:
 
Water and saline buffer
CAS number:
[83481-45-2]
Source:
 
Synthetic
Purity rate:
 
> 67 %
 
Reference
1. Jacobsen, R. B., et al. (1999) Critical residues influence the affinity and selectivity of alpha-conotoxin MI for nicotinic 
    acetylcholine receptors, Biochemistry. PubMed link
2. Gray, W. R., et al. (1983) Conotoxin MI. Disulfide bonding and conformational states, J Biol Chem. PubMed link
3. Papineni RV, et al. (2001) Site-specific charge interactions of alpha-conotoxin MI with the nicotinic acetylcholine
    receptor. J Biol Chem. PubMed link
 
 
α-Conotoxin GI / Alpha conotoxin GI
 
α-conotoxin GI has been isolated from the venom of the cone snail Conus geographus. This conotoxin acts on
postsynaptic membranes, which bind to the nicotinic acetylcholine receptor (nAChR). It Inhibits nAChR currents. The
highest affinity site is for the α/δ site on mouse muscle-derived BC3H-1 receptor, and the other site (α/γ site) on
nicotinic receptors from Torpedo californica electric organ.
 
Details
AA sequence:
 
Glu-Cys2-Cys3-Asn-Pro-Ala-Cys7-Gly-Arg-His-Tyr-Ser-Cys13-NH2
 
 
(Disulfide bonds between Cys2-Cys7, Cys3-Cys13)
Length (aa):
 
13
Formula:
C55H80N20O18S4
Molecular Weight:
 
1437. 61 Da
Appearance:
 
White lyophilized solid
Solubility:
 
Water and saline buffer
CAS number:
[76862-65-2]
Source:
 
Synthetic
Purity rate:
 
> 95 %
 
References
1. Groebe, D. R., et al. (1997) Determinants involved in the affinity of alpha-conotoxins GI and SI for the muscle subtype
    of nicotinic acetylcholine receptors, Biochemistry. PubMed link
 
 
α Conotoxin IMI / Alpha conotoxin IMI
 
α-conotoxin IMI has been isolated from the venom of the cone snail Conus imperialis. α-conotoxin IMI acts on
postsynaptic membranes, and binds onto and inhibits nicotinic acetylcholine receptors (nAChR). This toxin blocks
mammalian neuronal nAChRs (α3/ß2 > α7 > α3/ß4). It has no effect on nAChRs composed of α2/ß2, α3/ß2, α4/ß2, α2/ß4, α3/ß4, or α4/ß4 subunits. α-conotoxins IMI acts independently of voltage value. This toxin is highly active against the neuromuscular receptor in frog.
 
Details
AA sequence:
 
H-Gly-Cys2-Cys3-Ser-Asp-Pro-Arg-Cys8-Ala-Trp-Arg-Cys12-NH2
 
 
(Disulfide bonds between Cys2-Cys8and Cys3-Cys12)
Length (aa):
 
12
Formula:
C52H78N20O15S4
Molecular Weight:
 
1352. 92 Da
Appearance:
 
White lyophilized solid
Solubility:
 
Water and saline buffer
CAS number:
[156467-85-5]
Source:
 
Synthetic
Purity rate:
 
> 95 %
 
Reference
1. Ellison, M., et al. (2003) Alpha-conotoxins ImI and ImII. Similar alpha 7 nicotinic receptor antagonists act at different
    sites, J Biol Chem. PubMed link
2. Rogers, J. P., et al. (2000) Structure-activity relationships in a peptidic alpha7 nicotinic acetylcholine receptor
    antagonist, J Mol Biol. PubMed link
3. Johnson, D. S., et al. (1995) alpha-Conotoxin ImI exhibits subtype-specific nicotinic acetylcholine receptor blockade:
    preferential inhibition of homomeric alpha 7 and alpha 9 receptors, Mol Pharmacol. PubMed link
4. McIntosh, J. M., et al. (1994) A nicotinic acetylcholine receptor ligand of unique specificity, alpha-conotoxin ImI,
    J Biol Chem. PubMed link
 
 
Ordering informations
 
Catalog No.
Product Name
Size
12WAG001
Waglerin 1
    1 mg &  5 x 1 mg
08CON012
α-Conotoxin MI
     0.5 mg & 1.0 mg
  08CON005
α-Conotoxin GI
     0.5 mg & 1.0 mg
  08CON011
                           α-Conotoxin IMI
    0.5 mg & 1.0 mg
 
* 다른 사이즈나 bulk 공급도 가능하오니 별도로 문의하여 주십시오.
 
 
▣ 관련 페이지 ; Smartox Biotechnology
 
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