Biotech Support Group
 
작성일 : 13-07-12
[Biotech Support Group] Albumin depletion kits, AlbuSorb / AlbuVoid
Albumin depletion kits, AlbuSorb / AlbuVoid
 
 
Albusorb™
Albumin depletion kit from serum or plasma
 
 
다수의 전하를 갖는 elastomer의 표면에 단백질이 결합하는 성질을 이용하여 혈청이나 혈장에서 알부민을 제거하는 시약입니다. 시약을 추가하여 간단한 작업 만으로 정제가 완료됩니다. 또한 시료에 맞게 시약의 양을 조절 할 수 있기 때문에 경제적입니다.
 
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• Removes >90% albumin from 30 mg albumin/ml sample
• Affinity-type equivalence, virtually no cross-reactivity with other proteins
• Bind and elute procedure - simply weigh powder, condition the sample, centrifuge and/or filter, and recover the
  albumin depleted serum
• Economical new surface technology, not based on affinity chromatography
• Mild conditions maintain tertiary structure of proteins and simple transfer to secondary analysis
• The albumin depleted filtrate retains the enzymatic and biological activity
• Removes albumin from samples such as serum, plasma and from species including human, mouse, sheep, bovine, goat,
  rat, and calf
• The flow through fraction is compatible with LC-MS, activity based protein profiling and proteomic studies.
 
Albusorb™ is an albumin depletion reagent supplied as a kit with necessary buffers. With 25ul of starting sample, the yield
of albumin depleted serum protein is 0.1-0.2 milligrams. Albusorb™ binds albumin from serum or plasma and is
compatible with downstream proteomics methods such as protein array pixelation ,1D and 2D gel electrophoresis, LC/MS,
and MALDI-TOF MS. Samples are also prepared for biomarker discovery, toxicological studies for new drugs,
enzyme assays, protein profiling using SELDI analysis and cytokines research.
 
AlbuSorb™ binds to albumin, and serum proteins flow through. Removal of albumin allows enhanced detection of low
abundance proteins. AlbuSorb™ comes from a silica-based, separation platform utilizing a new combination of surface
microenvironments substituted with low molecular substrates that feature drug-binding motifs. Unlike immuno-affinity,
the surfaces utilized are disposable eliminating cycle to cycle variance and cross-contamination.
 
 
 
 
 
AlbuVoid™
Albumin Depletion and Low Abundance Protein Enrichment Kit from Serum, Plasma, Tissues And Culture Media
 
 
혈청이나 혈장에서 알부민제거하는 kit 입니다. 혈청이나 혈장에서 알부민분리하여 미량 단백질과 저분자 단백질을
농축 할 수 있습니다. 키트에는 필요한 버퍼 spin filter가 포함되어 있습니다.
 
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Albumin voids in flow-through >95%, with <30 minute bind/wash/elute protocol
Low abundance enrichment equivalent or better than hexa-peptides or antibodies
Disposable, cost-effective, no column regeneration or cross-contamination
Mild elution maintains tertiary structure and simple transfer to secondary analysis
The eluted fractions retain their enzymatic and biological activity
Removes albumin from many species including human, mouse, sheep, bovine, goat, rat, calf, cell culture.
The eluted fraction is compatible with LC-MS, activity based protein profiling and proteomic studies.
 
AlbuVoid™ is a albumin depletion reagent kit. It removes albumin from serum and plasma samples while concentrating low
abundance, and/or low molecular weight proteins. The AlbuVoid™ protocol uses mild buffers; the protocol conditions are so
gentle that native enzyme activity is retained in elution fractions. AlbuVoid™ considerably enhances resolution of
proteins below 50 kD, a limitation of alternate enrichment protocols.
 
AlbuVoid™ does not bind albumin. All other proteins(except albumin) in the sample binds to AlbuVoid™ and then you
can elute off all the proteins minus the albumin. Resulting in low abundance serum protein enrichment. It is ideal for
applications involving biomarker discovery, enzyme assays, toxicological studies for new drugs, protein profiling using
SELDI analysis, protein array pixelation ,1D and 2D gel electrophoresis, LC/MS, and MALDI-TOF MS and cytokines
research.
 
AlbuVoid™ derives from a silica-based library of individual mixed-mode polymeric ligands. The library was designed to
facilitate weak binding of proteins, allowing for rapid elution from the matrix without any foreknowledge of the variety of
proteins contained in the starting sample. Because of its specific binding properties, AlbuVoid™ depletes high abundance
proteins in serum like albumin while improving the resolution of less abundant serum proteins.
 
 
 
 
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Ordering informations
 
Catalog No.
Product Name
Size
A185-6
Albusorb™
4.3ml of 25µL serum samples processed
A185-18
13ml of 25µL serum samples processed
AVK-05
AlbuVoid™
5 preps, 200µL of samples can be processed per prep,
AVK-10
10 preps, 200µL of samples can be processed per prep,
AVK-50
50 preps, 200µL of samples can be processed per prep,
 
 
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