LACEseq for ribosome profiling
LACEseq kit uses IMMAGINA’s proprietary technology for selective sequencing of ribosome footprints
produced by the action of a nuclease generating RNA fragments with a 3’-phosphorylated end.
The kit is designed for a quick and accurate library preparation and sequencing of Ribosome Protected
LACEseq is the only all-inclusive solution for ribosome profiling experiments when combined with RiboLace
module 1 for active ribosome isolation and PAGExtTM for optimal RPFs recover.
• Selective sequencing of Ribosome protected fragments (RPFs)
• Reduced rRNA contamination
• Reduced sequencing deep needed for optimal Ribo-seq analysis
• Quick (2-day) high quality library preparation
• The only ALL INCLUSIVE solution when combined with RiboLace and PAGExtTM
LACEseq work flow
PAGE extracted ribosome protected fragments
LACEseq was developed to selectively sequence RPFs, for high quality Ribo-seq analysis. The final library
is 220 nt and Illumina adapter and index sequences are incorporated during library amplification.
As illustrated in fig.2, the complete LACEseq workflow comprises six steps: (1)RPF phosphorylation, (2)
adaptor ligation, (3)circularization, (4)Reverse transcription and (5,6)two PCR steps.
Indexing primers are provided with the LACEseq UDIplateTM(cat. number #iUD-12), for unique dual index
Following PCR amplification, purification, and validation, sequencing libraries may require size selection.
Size selection can be performed using PAGExt kit(cat.number #KGE-002) by IMMAGINA.
This product is also available in combined sets
UDI Plates LACEseq
PAGE Gel Extraction kit
RiboLace Module 1 kit
Cutadapt LACEseq supplemental resource and tips for success (PDF)
Data Sheet (PDF)