Glutathione Resin is designed for the affinity purification of proteins with a glutathione S-transferase (GST)
tag. The resin consists of reduced glutathione (GSH) covalently coupled to 6% cross-linked agarose, via a
10-carbon spacer arm. The resin has a binding capacity of >40mg GST/ml resin. Supplied as slurry in 20%
The resin, columns and binding/wash and elution buffers are available separately.
The spin columns are supplied with a resin bed volume of 0.2, 1 and 3ml with total column volumes of 1, 8
and 22ml respectively. Columns can be used as a spin format of gravity flow columns.
Also available in 96-well spin plate formats for processing up to 96 samples.
Our new HOOK™ GST Protein Purification kits are now available and include everything required for
purification of GST tagged proteins from yeast or bacteria, including lysis buffers, lytic enzymes, resin,
columns and binding, wash and elution buffers. Kits available are:
HOOK™ GST Protein Purification (Bacteria): Isolation from bacteria; optimized to yield up to 10mg/250ml
culture of soluble GST tagged protein.
HOOK™ GST Protein Spin Purification (Bacteria): Isolation from bacteria;optimized to yield ~1mg/50ml
culture of soluble GST tagged protein
HOOK™ GST Protein Purification (Yeast): Isolation from yeast; optimized to yield up to 10mg of soluble GST
HOOK™ GST Protein Spin Purification (Yeast): Isolation from yeast; optimized to yield up to 1mg of soluble
GST tagged protein.
Purified GST protein is also offered as a positive control for purification and Western blotting.
• For the purification of GST proteins
• High capacity (>40mg/ml)
• Bead size: 50-160μm
• Bead structure: 6% cross-linked agarose
• 10 carbon spacer arm.
• Affinity purification of proteins with a GST tag.
Instruction sheet (PDF)