HOOK¢â GST Protein Purification (Yeast)

HOOK¢â GST Protein Spin Purification kit allows for the rapid purification of soluble, GST tagged protein from
yeast cultures. The yeast are first lysed with Yeast PE LB¢â and LongLifeTM Zymolyase¢ç to release total
soluble protein, whilst maintaining the structure and activity of the protein. The GST tagged protein is purified
by passing the clarified lysate through prepacked columns.
Yeast-PE LB¢â is useful for extraction of soluble proteins from yeast cells. Yeast PE LB¢â is a proprietary
improvement on the Zymolyase¢ç based spheroplast preparation and extraction of soluble proteins from
yeast cells. Yeast PE LB¢â is based on organic buffering agents that utilize a mild non-ionic detergent and a
proprietary combination of various salts and agents to enhance extraction and stability ofproteins. A ready-
to-use Zymolyase¢ç preparation is also provided. Depending on application, additional agents such as
protease inhibitors may be added into Yeast PE LB¢â. The proprietary combination of this reagent provides a
simple and versatile method of yeast protein extraction. Yeast PE LB¢â eliminates the need for laborious
glass bead lysis of yeast cells.
HOOK¢â GST Protein Spin Purification kit is optimized to yield up to 10mg of soluble GST tagged protein, with
a purity of 80-90%, dependent on expression levels, resin type, conformation and solubility characteristics of
the protein.
For small scale, rapid GST protein isolation from yeastwe recommend our spin format kits.
Features;
• Isolate >10mg GST tagged proteins from yeast
• No sonication required, supplied with lysis buffer and enzyme
Application;
• For the isolation of GST tagged recombinant proteins from yeast
Instruction sheet (PDF)
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