HOOK™ GST Protein Purification (Bacteria)
HOOK™ GST Protein Purification kit allows for the purification of soluble, GST tagged protein from bacterial
cultures. The bacteria are first lysed with Bacterial PE LB™ and PE LB™-Lysozymeto release total soluble
protein,whilst maintaining the structure and activity of the protein.The GST tagged protein is purified by
passing clarified lysate through prepacked columns.
Bacterial- PELB™ kit has been developed for the extraction of soluble proteins from bacterial cells. It is a
proprietary improvement on the lysozyme based lysis, which allows extraction of soluble proteins and
concurrent removal of nucleic acids (DNA & RNA) released during cell lysis. The Bacterial-PE LB™ lysis
eliminates viscosity build-up, allowing effective clarification with lower centrifugal force.
HOOK™ GST Protein Purification kit is optimized to yield 10mg/250ml culture of soluble GST tagged protein,
with a purity of 80-90%, dependent on expression levels, resin type, conformation and solubility
characteristics of the protein.
For smaller scale, rapid GST protein isolation from bacteria we recommend our spin format kits.
• Isolate >10mg GST tagged proteins from ~250ml bacteria
• No sonication required, supplied with lysis buffer and enzyme
• For the isolation of GST tagged recombinant proteins from bacteria
Instruction sheet (PDF)