HOOK™ GST Protein Spin Purification (Bacteria)
HOOK™ GST Protein Spin Purification kit allows for the rapid purification of soluble, GST tagged protein from
bacterial cultures. The bacteria are first lysed with Bacterial PE LB™ and PE LB™-Lysozymeto release total
soluble protein, whilst maintaining the structure and activity of the protein.The GST tagged protein is purified
by affinity chromatography by adding 0.5ml glutathione resin to the clarified lysate. The resin is transferred to
a convenient spin column, where it is rapidlywashed and the GST protein is eluted.
Bacterial- PELB™ kit has been developed for the extraction of soluble proteins from bacterial cells. It is a
proprietary improvement on the lysozyme based lysis, which allows extraction of soluble proteins and
concurrent removal of nucleic acids (DNA & RNA) released during cell lysis. The Bacterial-PE LB™ lysis
eliminates viscosity build-up, allowing effective clarification with lower centrifugal force.
HOOK™ GST Protein Spin Purification kit is optimized to yield ~1mg/50ml culture of soluble GST tagged
protein, with a purity of 80-90%, dependent on expression levels, resin type, conformation and solubility
characteristics of the protein.
For larger scale GST protein isolation from bacteria we recommend our gravity flow kits for >10mg protein
from ~250ml bacteria.
• Isolate >1mg GST tagged proteins from ~50ml bacterial culture
• No sonication required, supplied with lysis buffer and enzyme
• Spin column format
• Spin column format for the isolation of GST-tagged recombinant proteins from bacteria
Instruction sheet (PDF)