Proteinase K Assay [Colorimetric]
Proteinase K Assay [Colorimetric] is designed for rapid and reproducible quantification of Proteinase K
activity. Proteinase K Assay [Colorimetric] is based on hydrolysis of denatured hemoglobin by proteinase K
which releases Folin positive amino acids and peptides. The amino acids and peptide react with Folin’s
reagent to give colored product which is measured at absorbance of 578nm.
Proteinase K is widely used in molecular biology for isolation and purification of nucleic acids from various
tissue and cell samples. The proteolytic activity of proteinase K helps in removing nucleases from the
sample, there by protecting DNA and RNA from degradation.
Recently, Proteinase K is widely used in COVID-19 virus diagnostic assays for processing samples such as
saliva, nasopharyngeal and oropharyngeal swabs. After proteinase K treatment the samples are heat treated
to inactivate proteinase K as well as inactivate any residual pathogens or viruses left in sample. Proteinase K
assay can help in optimizing sample processing step for the COVID-19 diagnostic assay.
• Rapid and reproducible
• Linear detection range: 5µg/ml- 100µg/ml
• Available in 100 assay tube format, adaptable to microwell assay format.
• Estimates proteinase K activity in various samples.
• Can be used in optimizing sample processing step for the COVID-19 diagnostic assay.
Instruction sheet (PDF)