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작성일 : 21-02-01
[ImmuSmol] HomoArginine ELISA kit

 

HomoArginine ELISA kit

 

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HomoArginine (hArg) is a basic non-essential amino acid that exerts beneficial effects on vascular

homeostasis, more likely through the increase of nitric oxide (NO) that can be explained through two

mechanisms since hArg i) serves as a precursor of NO and ii) favors the elevation of L-Arg - main substrate

of NO synthase - by inhibiting the enzyme Arginase.

 

The homoArginine (hArg) ELISA kit allows for the determination of hArg in serum and plasma samples

working with a sample volume of 20μL. The kit is easy to implement and well suited for both preclinical and

clinical studies.

 

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Typical standard curve of Homoarginine ELISA Standard curve obtained with the hArg ELISA kit. In

this competitive enzyme Immunoassay, optical density is inversely correlated with hArg levels within a linear

range of 0.32 - 12.5µM.

 

Specifications

Format;

96-well plate

Sample;

Serum & plasma  

Minimal sample volume;

20  µl

Species-reactivity;

React with all species

Standard range;

0.32 - 12.5 µM

Sensitivity;

125 nM

Specificity;

 

No significant cross-reactivity was observed with L-Arginine, Agmatine, L-Citruline, L-Ornithine, ADMA and SDMA.

Assay time;

Sample preparation 3h and ELISA overnight

Storage;

Store at 2 ~ 8°C for to 6 months.

 

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Cross-validation of Homoarginine ELISA and LC/MS data in human plasma sampleshArg was

quantified in human plasma samples from 40 healthy subjects either using IS-I-0500 ELISA kit or by liquid

chromatography–mass spectrometry (LC/MS). Correlation coefficient of 0.964 confirms the accuracy of the

immunoassay.

 

Instruction sheet (PDF)

 

References;

  1. Karetnikova et al. Is Homoarginine a Protective Cardiovascular Risk Factor?Arterioscler Thromb Vasc Biol. 2019

  2. Wetzel et al. L-Homoarginine supplementation prevents diabetic kidney damage.Physiol Rep. 2019

  3. Helm et al. Plasma Homoarginine Concentrations According to Use of Hormonal Contraception.Sci Rep. 2018

  4. Atzler et al. L-homoarginine and cardiovascular disease. Curr Opin Clin Nutr Metab Care. 2015

 

  

Ordering information

    

Catalog No.

Product Name

Size

IS-I-0500

HomoArginine ELISA kit

96 wells plate

 

 

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