AL Anacyte Laboratories UG
 
ÀÛ¼ºÀÏ : 13-06-20
[AL Anacyte Laboratories UG] Á¾¾ç µîÀÇ biomolecules¸¦ °£´ÜÈ÷ ¾ÈÁ¤È­ÇÏ´Â ½Ã¾à
Store your cells in CellCover and protect your RNA integrity, CellCover
 
Á¾¾ç µîÀÇ biomolecules¸¦ °£´ÜÈ÷ ¾ÈÁ¤È­ÇÏ´Â ½Ã¾à, CellCover
 
 
ÇöÀç ¼ö¼ú ÈÄ, ºÐ¸®µÈ Á¶Á÷ÀÌ ½Ç¿ÂÀ̳ª PBS¿¡ ÀúÀåµÇ°Å³ª ±âŸ ´Ù¸¥ ¹æ¹ýÀ¸·Î º¸°üµÇ´Âµ¥ ÀÌ´Â RNA/Protein expression
pattern¿¡ º¯È­¸¦ ÁÙ ¼ö°¡ ÀÖ½À´Ï´Ù.
CellCover´Â »ç¶÷À̳ª µ¿¹°ÀÇ Á¾¾çÁ¶Á÷, ¹éÇ÷±¸¼¼Æ÷, ¹è¾ç¼¼Æ÷(adherent, suspension, spheroids) µîÀÇ DNA, RNA ¹× proteinÀ» one-step(½Ã·á·®ÀÇ 5¹è·® ÷°¡·Î ¿Ï·á) À¸·Î ºü¸£°Ô ¾ÈÁ¤È­, º¸Á¸ÇÏ´Â ½Ã¾àÀÔ´Ï´Ù. ¾×ü Áú¼Ò¸¦ »ç¿ëÇÏÁö ¾Ê´õ¶óµµ,
CellCover¿¡ ³ëÃâµÈ ¼¼Æ÷µéÀº ´ë»ç»óŰ¡ ¸ØÃß°Ô µÇ¾î chemical degradation ¾øÀÌ,DNA, RNA ¹× proteinÀ» ¾ÈÁ¤È­½ÃÄÑ 
½Ã·á¸¦ ¸î ÀÏ µ¿¾È º¸Á¸ ÇÒ ¼ö ÀÖ½À´Ï´Ù. CellCover´Â enzymatic hydrolysis¸¦ shielding molecules·Î ¾ÈÁ¤È­ÇÕ´Ï´Ù.  CellCoverÀÇ ¶Ç ´Ù¸¥ ÀåÁ¡Àº ÇÙ»êÀ̳ª ´Ü¹éÁúÀÇ 2Â÷, 3Â÷ ±¸Á¶°¡ À¯ÁöµÇ°Ô Çϱ⠶§¹®¿¡, CellCover¸¦ ÀÌ¿ëÇÑ Á¶Á÷À̳ª ¼¼Æ÷µéÀº in-vivo »óÅÂ¿Í °°µµ·Ï DNA, RNA ¹× proteinÀ» À¯Áö½ÃŲ´Ù´Â °ÍÀÔ´Ï´Ù. CellCover·Î ó¸®ÇÑ ½Ã·á´Â ½Ç¿Â(25 ¡É)¿¡¼­ ÇÏ·ç, 4 ¡É¿¡¼­ 4 ÁÖ±îÁö ÀúÀå ÇÒ ¼ö ÀÖÀ¸¸ç, ½Ã·á´Â ¸é¿ª¿°»ö, flow cytometry, in situ hybridization µî¿¡ Àû¿ë ÇÒ ¼ö ÀÖ½À´Ï´Ù.
 
The Benefits
If treated with CellCover liquid freezing, cells grown on almost any substrate maintain in vivo morphology without
chemical crosslinking of biomolecules. Compared to conventional formaldehyde fixation, formalin sensitive epitopes are
readily accessible for antibodies in immunohistochemical experiments. A unique and special feature of CellCover liquid
freezing is that you can perform additional molecular analysis on cells already analysed on morphological or molecular level: DNA, RNA (with high RINs) or protein can be isolated for subsequent applications after initial IHC, ICC or FACS analyses.
Even integrity of high molecular weight RNA is protected (e.g precurser rRNA, which are normally degradated after
applying commonly used standards procedures) - and there is no need to hurry!
 
 Å¬¸¯ÇÏ½Ã¸é ´ÝÈü´Ï´Ù.À̹ÌÁö ÀúÀåÀ» ¿øÇÏ½Ã¸é ¸¶¿ì½º ¿À¸¥ÂÊŬ¸¯ÈÄ '´Ù¸¥À̸§À¸·Î ÀúÀå'À» Çϼ¼¿ä
 
Time course: Cultured cells (SKMel-28) stored for 12 days in CellCover. RNA integrity was mesuared with Agilent
Bioanalyzer.  Results show a stable RIN(RNA integrity number) value of 10 from first till last day of the time course.
 
 
Ŭ¸¯ÇÏ½Ã¸é ´ÝÈü´Ï´Ù.À̹ÌÁö ÀúÀåÀ» ¿øÇÏ½Ã¸é ¸¶¿ì½º ¿À¸¥ÂÊŬ¸¯ÈÄ '´Ù¸¥À̸§À¸·Î ÀúÀå'À» Çϼ¼¿ä                    Å¬¸¯ÇÏ½Ã¸é ´ÝÈü´Ï´Ù.À̹ÌÁö ÀúÀåÀ» ¿øÇÏ½Ã¸é ¸¶¿ì½º ¿À¸¥ÂÊŬ¸¯ÈÄ '´Ù¸¥À̸§À¸·Î ÀúÀå'À» Çϼ¼¿ä
(Left) CellCover fixed cell on glass slide. Positive IHC signal for formalin-sensitive Epitope Vimentin.
(Right) Formalin fixed cell on glass slide. No IHC signal for formalin-sensitive Epitope Vimentin.
 
 
Ŭ¸¯ÇÏ½Ã¸é ´ÝÈü´Ï´Ù.À̹ÌÁö ÀúÀåÀ» ¿øÇÏ½Ã¸é ¸¶¿ì½º ¿À¸¥ÂÊŬ¸¯ÈÄ '´Ù¸¥À̸§À¸·Î ÀúÀå'À» Çϼ¼¿ä                    Å¬¸¯ÇÏ½Ã¸é ´ÝÈü´Ï´Ù.À̹ÌÁö ÀúÀåÀ» ¿øÇÏ½Ã¸é ¸¶¿ì½º ¿À¸¥ÂÊŬ¸¯ÈÄ '´Ù¸¥À̸§À¸·Î ÀúÀå'À» Çϼ¼¿ä
(Left) Primary cells shows high cellular integrity by FACS analysis.
(Right) Gated cells (R1) with red and green fluorescence signals.
 
 
The opportunities
 
These unique properties now enable new types of analyses of cellular functions. With CellCover liquid freezing,
subpopulations of cells can be separated according to known properties by flow cytometry and subsequently analysed for
gene expression profiles on RNA or on protein-level. Thus, with CellCover liquid freezing it is easy to link transcriptome
and proteome of a given cell population. In comparison to commonly used molecular biological methods, CellCover adds
following new analytical tools to bio-molecular research:
 
• Stabilize RNA and Protein simultaneously for several days, without doubt of molecule integrity
• Reliable analysis HMW-RNA and low stability enzymes even after day of storage
• Apply immunohistochemistry on formalin sensitive epitopes
• Apply in situ hybridization for analysis of gene expression
• Store cells conveniently for several days without change of protein or RNA expression profile or change in cells  
  morphology as found with methanol
• Isolate subpopulations by flow cytometry based on protein labelling and perform subsequent transcriptome and/or
  proteome (MALDI) analyses.  
 
 
FAQ (PDF)
 
 
Application quides
 
 Cytology                                         Flow Cytometry and Immuno Fluorescence
Ŭ¸¯ÇÏ½Ã¸é ´ÝÈü´Ï´Ù.À̹ÌÁö ÀúÀåÀ» ¿øÇÏ½Ã¸é ¸¶¿ì½º ¿À¸¥ÂÊŬ¸¯ÈÄ '´Ù¸¥À̸§À¸·Î ÀúÀå'À» Çϼ¼¿ä                          Å¬¸¯ÇÏ½Ã¸é ´ÝÈü´Ï´Ù.À̹ÌÁö ÀúÀåÀ» ¿øÇÏ½Ã¸é ¸¶¿ì½º ¿À¸¥ÂÊŬ¸¯ÈÄ '´Ù¸¥À̸§À¸·Î ÀúÀå'À» Çϼ¼¿ä
 
 
Ordering informations
 
Catalog No.
Product Name
Size
800-125
CellCover
125 ml
800-250
CellCover
250 ml