Á¾¾ç µîÀÇ biomolecules¸¦ °£´ÜÈ÷ ¾ÈÁ¤ÈÇÏ´Â ½Ã¾à, CellCover
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½Ã·á¸¦ ¸î ÀÏ µ¿¾È º¸Á¸ ÇÒ ¼ö ÀÖ½À´Ï´Ù. CellCover´Â enzymatic hydrolysis¸¦ shielding molecules·Î ¾ÈÁ¤ÈÇÕ´Ï´Ù. CellCoverÀÇ ¶Ç ´Ù¸¥ ÀåÁ¡Àº ÇÙ»êÀ̳ª ´Ü¹éÁúÀÇ 2Â÷, 3Â÷ ±¸Á¶°¡ À¯ÁöµÇ°Ô Çϱ⠶§¹®¿¡, CellCover¸¦ ÀÌ¿ëÇÑ Á¶Á÷À̳ª ¼¼Æ÷µéÀº in-vivo »óÅÂ¿Í °°µµ·Ï DNA, RNA ¹× proteinÀ» À¯Áö½ÃŲ´Ù´Â °ÍÀÔ´Ï´Ù. CellCover·Î ó¸®ÇÑ ½Ã·á´Â ½Ç¿Â(25 ¡É)¿¡¼ ÇÏ·ç, 4 ¡É¿¡¼ 4 ÁÖ±îÁö ÀúÀå ÇÒ ¼ö ÀÖÀ¸¸ç, ½Ã·á´Â ¸é¿ª¿°»ö, flow cytometry, in situ hybridization µî¿¡ Àû¿ë ÇÒ ¼ö ÀÖ½À´Ï´Ù.
If treated with CellCover liquid freezing, cells grown on almost any substrate maintain in vivo morphology without
chemical crosslinking of biomolecules. Compared to conventional formaldehyde fixation, formalin sensitive epitopes are
readily accessible for antibodies in immunohistochemical experiments. A unique and special feature of CellCover liquid
freezing is that you can perform additional molecular analysis on cells already analysed on morphological or molecular level: DNA, RNA (with high RINs) or protein can be isolated for subsequent applications after initial IHC, ICC or FACS analyses.
Even integrity of high molecular weight RNA is protected (e.g precurser rRNA, which are normally degradated after
applying commonly used standards procedures) - and there is no need to hurry!
Time course: Cultured cells (SKMel-28) stored for 12 days in CellCover. RNA integrity was mesuared with Agilent
Bioanalyzer. Results show a stable RIN(RNA integrity number) value of 10 from first till last day of the time course.
(Left) CellCover fixed cell on glass slide. Positive IHC signal for formalin-sensitive Epitope Vimentin.
(Right) Formalin fixed cell on glass slide. No IHC signal for formalin-sensitive Epitope Vimentin.
(Left) Primary cells shows high cellular integrity by FACS analysis.
(Right) Gated cells (R1) with red and green fluorescence signals.
These unique properties now enable new types of analyses of cellular functions. With CellCover liquid freezing,
subpopulations of cells can be separated according to known properties by flow cytometry and subsequently analysed for
gene expression profiles on RNA or on protein-level. Thus, with CellCover liquid freezing it is easy to link transcriptome
and proteome of a given cell population. In comparison to commonly used molecular biological methods, CellCover adds
following new analytical tools to bio-molecular research:
• Stabilize RNA and Protein simultaneously for several days, without doubt of molecule integrity
• Reliable analysis HMW-RNA and low stability enzymes even after day of storage
• Apply immunohistochemistry on formalin sensitive epitopes
• Apply in situ hybridization for analysis of gene expression
• Store cells conveniently for several days without change of protein or RNA expression profile or change in cells
morphology as found with methanol
• Isolate subpopulations by flow cytometry based on protein labelling and perform subsequent transcriptome and/or
proteome (MALDI) analyses.
Application quides
Cytology Flow Cytometry and Immuno Fluorescence
Ordering informations
Catalog No. |
Product Name |
Size |
800-125 |
CellCover |
125 ml |
800-250 |
CellCover |
250 ml |