Cellagen Technology LLC
 
ۼ : 12-05-05
[Cellagen Technology LLC] Glowth Factor, Recombinant human bFGF & Wnt-3a
Glowth Factor, Recombinant human bFGF & Wnt-3a
 
 
Product Name : Recombinant human bFGF
 
bFGF is a critical component of embryonic stem cell culture medium to maintain cells in an undifferentiated state.

 
Technical information
 
a. Expression Source :
E. coli.
 
b. Sequence :
Pro143 – Ser288, Accession # NP_001997
 
c. Endotoxin Level :
<1.0 EU per 1 g of the protein by the LAL method.
 
d. Purity :
>95%, by SDS-PAGE under reducing conditions and visualized by Coomassie Brilliant Blue stain.
 
e. Predicated Molecular Weight :
16 kDa
 
 
Biological Activity :
 
Basic FGF, also known as FGF-2, has a wide range of biological activities. FGF basic stimulates the proliferation of all cells
of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. FGF basic induces neuron
differentiation, survival, and regeneration.

Basic FGF has been used as a essential component of embryonic stem cell culture medium to maintain cells in an
undifferentiated state.
 
 
Ordering information ;
 
1. 50 ug
2. 500 ug
 

 
Product Name : Wnt-3a

Recombinant Wnt3a promotes proliferation of committed stem cell lineages in vitro, and may help maintain the cells in an undifferentiated state

Technical information

a. Expression Source : Chinese Hamster Ovary cell line
 
b. Sequence : Ser19 – Lys352, Accession # NP_149122
 
c. Endotoxin Level : <1.0 EU per 1 g of the protein by the LAL method.
 
d. Purity : >85%, by SDS-PAGE under reducing conditions and visualized by Coomassie Brilliant Blue stain.

e. Predicated Molecular Weight : 38 kDa
 
 
Biological Activity

Wnt-3a is one of about 19 vertebrate members of the Wingless type MMTV integration site (Wnt) family of highly
conserved cysteinerich secreted glycoproteins important for normal developmental processes.

Wnt-3a promotes proliferation of committed stem cell lineages in vitro, and may help maintain the cells in an
undifferentiated state. For example, Wnt-3a can induce self-renewal of hematopoietic stem cells, allowing expansion
without further differentiation (Nusse R, 2008, Cell Res)

 
Ordering information ;
 
1. 10 ug
2. 100 ug