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Kynurenic acid ELISA kit
Kynurenic acid (KYNA) is a downstream metabolite produced along the Kynurenine Pathway well-known to
possess several neuromodulatory functions, as well as immuno-modulatory properties. Measurement of
KYNA in biological samples has been presented as a possible biomarker in several diseases including
neurological or psychiatric disorders.
The Kynurenic Acid ELISA kit is optimized for the quantitation of Kynurenic Acid (KYNA) within serum
samples. This easy-to-use competitive assay is well-suited for both small and large series of samples.
Working with a 50µL sample volume, this assay enables pre-clinical and clinical sample testings.
Cross-validation of Kynurenic Acid ELISA and LC/MS data in human serum samples Human
serum samples (n=40) were processed for Kynurenic Acid measurement by mean of both KYNA ELISA kit
and liquid chromatography–mass spectrometry (LC/MS). As depicted, results obtained with ELISA and
LC/MS are highly correlated (R Squared=0,9902).
Typical standard curve of Kynurenic acid ELISA Example of standard curve obtained with the KYNA
ELISA Kit. In this competitive ELISA, optical density is invertly correlated with Kynurenic Acid levels.
Specifications
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Format;
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96-well plate
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Sample;
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Serum
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Minimal sample volume;
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50 µl
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Species-reactivity;
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React with all species
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Standard range;
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1.4 ~ 74 ng/ml
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Sensitivity;
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0.53 ng/ml
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Specificity;
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No significant cross-reactivity was observed with Kynurenic Acid analogs such as Quinolinic Acid, Xanthurenic acid, Kynurenine, Picolinic acid, 3Hydroxy-Anthranilic Acid.
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Assay time;
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Sample derivatization 90 min, ELISA overnight
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Storage;
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Store at 2 ~ 8°C for to 6 months.
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Kynurenic acid (KYNA) is a metabolite produced along the Kynurenine Pathway Kynurenic acid
(KYNA) is a downstream metabolite produced along the Kynurenine Pathway well-known to possess several
neuromodulatory functions, as well as immuno-modulatory properties.
Instruction sheet (PDF)
References;
- Pedraz-Petrozzi et al. Effects of inflammation on the kynurenine pathway in schizophrenia - a systematic review. J Neuroinflammation. 2020
- Achtyes et al. Inflammation and kynurenine pathway dysregulation in post-partum women with severe and suicidal depression. Brain Behav Immun. 2020
- Walczak et al. Kynurenic acid and cancer: facts and controversies. Cell Mol Life Sci. 2019
- Lim et al. Kynurenine pathway metabolomics predicts and provides mechanistic insight into multiple sclerosis progression. Sci Rep. 2017
- Plitman et al. Kynurenic Acid in Schizophrenia: A Systematic Review and Meta-analysis. Schizophr Bull. 2017
- Wirthgen et al. Kynurenic Acid: The Janus-Faced Role of an Immunomodulatory Tryptophan Metabolite and Its Link to Pathological Conditions. Front Immunol. 2017
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작성일 : 20-12-23
