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작성일 : 20-12-23
[ImmuSmol] Kynurenic acid ELISA kit

 

Kynurenic acid ELISA kit

 

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Kynurenic acid (KYNA) is a downstream metabolite produced along the Kynurenine Pathway well-known to

possess several neuromodulatory functions, as well as immuno-modulatory properties. Measurement of

KYNA in biological samples has been presented as a possible biomarker in several diseases including

neurological or psychiatric disorders.

 

The Kynurenic Acid ELISA kit is optimized for the quantitation of Kynurenic Acid (KYNA) within serum

samples. This easy-to-use competitive assay is well-suited for both small and large series of samples.

Working with a 50µL sample volume, this assay enables pre-clinical and clinical sample testings.

     

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Cross-validation of Kynurenic Acid ELISA and LC/MS data in human serum samples Human

serum samples (n=40) were processed for Kynurenic Acid measurement by mean of both KYNA ELISA kit

and liquid chromatography–mass spectrometry (LC/MS). As depicted, results obtained with ELISA and

LC/MS are highly correlated (R Squared=0,9902).

 

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Typical standard curve of Kynurenic acid ELISA Example of standard curve obtained with the KYNA

ELISA Kit. In this competitive ELISA, optical density is invertly correlated with Kynurenic Acid levels.

 

Specifications

Format;

96-well plate

Sample;

Serum

Minimal sample volume;

50 µl

Species-reactivity;

React with all species

Standard range;

1.4 ~ 74 ng/ml

Sensitivity;

0.53 ng/ml

Specificity;

 

 

No significant cross-reactivity was observed with Kynurenic Acid analogs such as Quinolinic Acid, Xanthurenic acid, Kynurenine, Picolinic acid, 3Hydroxy-Anthranilic Acid.

Assay time;

Sample derivatization 90 min, ELISA overnight

Storage;

Store at 2 ~ 8°C for to 6 months.

 

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Kynurenic acid (KYNA) is a metabolite produced along the Kynurenine Pathway Kynurenic acid

(KYNA) is a downstream metabolite produced along the Kynurenine Pathway well-known to possess several

neuromodulatory functions, as well as immuno-modulatory properties.

 

Instruction sheet (PDF)

 

 

References;

  1. Pedraz-Petrozzi et al. Effects of inflammation on the kynurenine pathway in schizophrenia - a systematic review. J Neuroinflammation. 2020
  2. Achtyes et al. Inflammation and kynurenine pathway dysregulation in post-partum women with severe and suicidal depression. Brain Behav Immun. 2020
  3. Walczak et al. Kynurenic acid and cancer: facts and controversies. Cell Mol Life Sci. 2019
  4. Lim et al. Kynurenine pathway metabolomics predicts and provides mechanistic insight into multiple sclerosis progression. Sci Rep. 2017
  5. Plitman et al. Kynurenic Acid in Schizophrenia: A Systematic Review and Meta-analysis. Schizophr Bull. 2017
  6. Wirthgen et al. Kynurenic Acid: The Janus-Faced Role of an Immunomodulatory Tryptophan Metabolite and Its Link to Pathological Conditions. Front Immunol. 2017

 

 

Ordering information

    

Catalog No.

Product Name

Size

IS-I-0200

Kynurenic acid ELISA kit

96 wells plate

 

 

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