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작성일 : 20-12-01
[Profoldin] SARS-CoV-2 RNA Polymerase (RdRp) Assay kits
SARS-CoV-2 RNA Polymerase (RdRp) Assay for Drug Discovery

 

SARS-CoV-2 RNA Polymerase (RdRp) Assay for Drug Discovery

    

 

The COVID-19 pandemic is caused by infection of SARS-CoV-2 or severe acute respiratory syndrome

coronavirus 2, a positive-sense single-stranded RNA virus. The RNA dependent RNA polymerase (RdRp) is

a key drug target for this deadly virus. The SARS-CoV-2 RdRP assay is based on detection of the RNA

molecules synthesized by the RNA polymerase in the presence of an RNA template and NTPs. The assay

can be performed in a 384-well or 96-well plate format for tests of the enzyme activities of the virus RNA

polymerase (SARS-CoV-2 RdRp) and high throughput screening of inhibitors.   

 

 

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The SARS-CoV-2 RNA polymerase assay kit-100 (Catalog No.S2RPA100K)includes 300 µl of 10 x Buffer,

50 µl of 50 x RNA template, 60 µl of 50 x  mM NTPs (ATP, UTP, GTP, CTP) and 700 µl of 10 x fluorescence

dye. It is for 100 assays of SARS-CoV-2 RNA polymerase in a 384-well plate format or 50 assays in a 96-

well plate format. The assay kit includes all reagents except the enzyme.

 

The SARS-CoV-2 RNA polymerase assay kit plus-20 (Catalog No.S2RPA020KE) includes 75 µl of 10 x Buffer,

10 µl of 50 x RNA template, 12 µl of 50 x NTPs (ATP, UTP, GTP, CTP), 150 µl of 10 x fluorescence dye and

10 µl of 50 x SARS-CoV-2 RdRp.  It is for 20 assays of SARS-CoV-2 RNA polymerase in a 384-well plate

format or 10 assays in a 96-well plate format.  

 

The SARS-CoV-2 RNA polymerase assay kit plus-100 (Catalog No.S2RPA100KE)includes 300 µl of 10 x

Buffer, 50 µl of 50 x RNA template, 60 µl of 50 x  mM NTPs (ATP, UTP, GTP, CTP), 700 µl of 10 x

fluorescence dye and  50 µl of 50 x SARS-CoV-2 RdRp.  It is for 100 assays of SARS-CoV-2 RNA

polymerase in a 384-well plate format or 50 assays in a 96-well plate format.

 

 

 

Ordering informations

   

Catalog No.

Product Name

Size

S2RPA100K

SARS-CoV-2 RNA polymerase assay kit-100

100 assays

S2RPA020KE

SARS-CoV-2 RNA polymerase assay kit plus-20

20 assays

S2RPA100KE

SARS-CoV-2 RNA polymerase assay kit plus-100

100 assays

 

 

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