Gel-shift single-strand DNA binding assay
Single-strand DNA-binding protein (SSB) binds to single-stranded DNA (ssDNA) molecules or a single-
stranded region of a double strand-DNA in a biological process such as DNA replication, recombination, or
repair. For example, DNA helicase unwinds double-strand DNA to form single-strand DNA. E. coli SSB forms
homotetramer; each subunit has a molecular weight of 19 kDa. The Gel Shift Single-strand DNA Binding
Assay is designed for analysis of SSB DNA-binding activity using a conventional acrylamide
electrophoresis. The SSB DNA-binding activity is visualized by the DNA band shift. The SSB-bound DNA
migrates slower than the free DNA.
The Gel Shift Single-strand DNA Binding Assay Kit (Catalog No. GSSB100K)includes 150 µl of 10 x Buffer,
150 µl of 10 x ssDNA (a 45-mer single-strand DNA) and 100 µl of 4 x gel loading buffer.
The Gel Shift Single-strand DNA Binding Assay Kit Plus (Catalog No. GSSB100KE)includes 150 µl of 10 x
Buffer, 150 µl of 10 x ssDNA, 150 µl of 2 mg/ml E. coli single-strand DNA binding protein SSB and 100 µl of
4 x gel loading buffer.