작성일 : 20-10-30
[Invent Biotechnologies, Inc.] Minute™ Plant Golgi Apparatus Enrichment Kit


Minute™ Plant Golgi Apparatus Enrichment Kit


    클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요


The plant Golgi apparatus plays an important role in biosynthesis of cellular structural components and

protein trafficking. However proteomic characterization of the Golgi has been hindered by limited

methodologies for obtaining enriched/purified Golgi fraction. Traditional method for isolating Golgi apparatus

depends upon the use of density centrifugation, which is tedious and time consuming. Large amount of

starting material is usually required. Plant Golgi stacksare notoriously labile and loss of stack integrity can

occur if harsh homogenization is used. This means that assessment of purity by morphological means such

as electron microscopy is difficult. This kit is designed to overcome certain disadvantages of traditional

methods by employing spin column-based homogenization, differential centrifugation and selective

precipitation for enrichment of the Golgi. The protocol is simple and straight forward and requires only

milligram amount of starting material. Significantenrichment of Golgi fraction (2-3 folds) can be obtained.


 Enrichment of Golgi Apparatus from Plant Leaves

    클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

A. Ponceau S stained blot. B. Western blot with ER (BIP2), Golgi (Arf1) and histone 3 (H3) marker antibodies.

The antibodies were purchased from Agrisera (Vannas, Sweden). Lanes 1 & 2: total protein from A. thaliana

and N. tabacum; Lanes 3 & 5: enriched Golgi fractions from A. thaliana and N. tabacum, respectively.



Instruction sheet (PDF)



Ordering information


Catalog No

Product Name

Kit components



Minute™ Plant Golgi Apparatus Enrichment Kit

Buffer A

10 ml

Buffer B

1.2 ml

Buffer C

4 ml

Buffer D

0.5 ml

Filter Cartridges with Collection tube

20 units

Plastic Rods

2 units

* For 20 preps



▣ 관련 페이지 ; Invent Biotechologies, Inc.