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작성일 : 20-10-30
[Invent Biotechnologies, Inc.] Minute™ Plant ER Enrichment Kit

 

Minute™ Plant ER Enrichment Kit

 

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Isolation and enrichment of plant endoplasmic reticulum (ER) is essential for elucidation of ER related cellular

functions. Traditional method for ER isolation is tedious and time consuming.  In animal cells, ER is closely

associated microtubules and Golgi apparatus are clustered at microtubule organizing center. In plant cells,

the ER is associated with actin microfilaments and no microtubule organizing center is found near the nuclei.

Due to structural characteristics of plan ER, itis much more difficult to isolate/enrich plant ER than that of

animal samples. Minute™ plant ER enrichment kit was specially designed for plants. One to three-fold

enrichment of ER fraction can be obtained from plant leave samples in about 1 hour. The enriched ER is

essentially free from Golgi apparatus contamination. The enriched ER fraction can be used in plant protein

trafficking studies.

 

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A. Ponceau S Stained Blot; B. Western blot with Anti-ER marker antibody (BIP2, Agrisera, Vannas, Sweden.

AS 09481). Lanes 1 & 2: B. napus; Lanes 3 & 4: N. tabacum; Lanes 5 & 6: A. thaliana. Lanes 1, 3 & 5: Total

Protein; Lanes 2, 4 & 6: ER fractions.

 

 

Instruction sheet (PDF)

 

 

Ordering information

     

Catalog No

Product Name

Kit components

Amount

PR-048

Minute™ Plant ER Enrichment Kit

Buffer A

20 ml

Buffer B

1.2 ml

Buffer C

10 ml

Plastic Rods

2 ea

Filter Cartridges

20 units

2 ml Collection Tube

20 units

* For 20 preps

 

 

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