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작성일 : 20-09-24
[HansaBioMed Life Sciences] RNA basic kit, for EV RNA Isolation

 

RNA basic kit, for EV RNA Isolation

 

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Exosomes shuttle functional RNA molecules in the target cell. Increasing evidence suggests a role for

exosome-derived miRNAs in the development and/or progression of specific human diseases. Pathogenic

miRNAs might be exploited as novel therapeutic targets or disease biomarkers in complex diseases,

including cancer. In fact, miRNAs seem to play critical roles as transcriptional and post-transcriptional

regulatorsof epigenetic mechanisms and cell processes and have been linked to the etiology, progression

and prognosis of cancer. Similar miRNA expression patterns between tumor tissue samples and circulating

exosomes have been observed.

HBM has developed optimized solutions for the efficient extraction of high-quality total RNA (miRNA and

mRNAs) from the overall exosomes and microvesicle population or from tumor-specific exosome

subpopulation, which helps to facilitate the identification of tumor miRNA or mRNAs signatures from human

biofluids or cell culture media.

 

RNA Basic Kit allows RNA extraction from exosomes pre-isolated with different methods (ultracentrifugation,

chemical precipitation, immunocapture, size-chromatography etc).

 

Sample type:

 

Plasma, serum, urine, cell culture media pre-isolated exosomes.

Starting material:

 

Kit allows RNA extraction from exosomes pre-isolated from different samples

 

 

(biofluids or cell culture media).

Specificity:

 

Kit provides high yield and quality of RNA from pre-isolated vesicles. RNA is

 

 

suitable for multiple downstream analyses.

Shelf life:

 

24 months

      

Kit components;

  

RNA Washing buffer:

 

1 bottle (10 ml, 25 reactions)

Lysis buffer:

 

1 bottle (19 ml, 25 reactions)

Elution buffer:

 

1 vial (1ml)

Columns:

 

27 columns/25 reactions

Elution tubes:

 

27 tubes/25 reactions

RNA basic kit does not contain immunobeads for exosome isolation. It is designed for exosome total RNA

(miRNA + mRNAs) from pre-isolated exosomes. Compatible with exosome isolated via ultracentrifuge,

chemical precipitation, size chromatography, immunocapture etc.

The kit contains all the reagents necessary for RNA purification, including RNA binding columns for total RNA

capture.

   

  

  Data sheet (PDF)

 

Ordering information

     

Catalog No.

Product Name

Size

HBM-RNA-B25

RNA Basic Kit

25 reactions

 

 

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